Literature DB >> 3539722

Preparation of Clostridium septicum antigen for hyperimmunization of horses using a dialyzed culture.

Z Hnátková, B Vraný, J Hnátek, A Lettl.   

Abstract

The preparation of toxic cultures of Clostridium septicum is described, using an apparatus with a straight dialysis tubing, where the medium is filled both into the nutrition and cultivation space of the apparatus. Using the cultivation to nutrition volume rate 1:2, mean titre of lethal antigen in filtrates 3.86 limes mortis per mL and 300 dosis lethalis minima per Lm was obtained in comparison with the values of 2.22 and 150 respectively in flask filtrates. Native filtrates of dialyzed cultures were better antigens for hyperimmunization of horses than the culture filtrates from flasks.

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Year:  1986        PMID： 3539722     DOI： 10.1007/BF02936603

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  8 in total

1.  [STUDIES ON CLOSTRIDIUM BOTULINUM TYPE E. 3. PRODUCTION OF HIGHLY ACTIVE TOXIC BOTULINUM ANTIGENS IN CELLOPHANE BAG CULTURES].

Authors:  H MEISEL; H ALBRYCHT; D RYMKIEWICZ; A SWITALSKA; P TREMBOWLER
Journal:  Med Dosw Mikrobiol       Date:  1964

2.  Clostridium botulinum type E toxin and toxoid.

Authors:  A L BARRON; G B REED
Journal:  Can J Microbiol       Date:  1954-10       Impact factor: 2.419

3.  Preparation of antigens and vaccines against gas gangrene.

Authors:  A LETTL; B BLASKO; J HAZA
Journal:  J Hyg Epidemiol Microbiol Immunol       Date:  1962

4.  Studies on immunity to toxins of Clostridium botulinum. VI. Purification and detoxification of type D toxin and the immunological response to toxoid.

Authors:  M A CARDELLA; J T DUFF; B H WINGFIELD; C GOTTFRIED
Journal:  J Bacteriol       Date:  1960-03       Impact factor: 3.490

5.  The growth of Brucella abortus strain 19 in aerated dialysed media.

Authors:  M STERNE
Journal:  J Gen Microbiol       Date:  1958-06

6.  A simple method for obtaining highly potent tetanus toxin.

Authors:  W KOCH; D KAPLAN
Journal:  J Immunol       Date:  1953-01       Impact factor: 5.422

7.  [Production in cellophane bags of tetanus toxins containing a minimum of 600,000 lethal guinea pig doses per milliliter].

Authors:  V FREDETTE; G VINET
Journal:  Can J Med Sci       Date:  1952-04

8.  A new method for the large-scale production of high-titre botulinum formol-toxoid types C and D.

Authors:  M STERNE; L M WENTZEL
Journal:  J Immunol       Date:  1950-08       Impact factor: 5.422
  8 in total
  2 in total

Review 1.  Production of toxic antigens in dialyzed cultures of microorganisms.

Authors:  B Vraný; Z Hnátková; A Lettl
Journal:  Folia Microbiol (Praha)       Date:  1988       Impact factor: 2.099

Review 2.  Vaccine Production to Protect Animals Against Pathogenic Clostridia.

Authors:  Nicolas E Zaragoza; Camila A Orellana; Glenn A Moonen; George Moutafis; Esteban Marcellin
Journal:  Toxins (Basel)       Date:  2019-09-11       Impact factor: 4.546
  2 in total

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