Literature DB >> 3539083

Improvement of prolactin immunolabelling in osmium-fixed acrylic-embedded pituitary gland.

C A Maldonado, A Aoki.   

Abstract

Immunolabelling of prolactin (PRL) with protein A-colloidal gold complex and tissue fine structure were enhanced after postfixation of pituitary gland with osmium tetroxide and embedment in acrylic momers (LR White). Thin sections were treated with sodium metaperiodate before immunocytochemistry. An intense PRL labelling was detected in secretory granules, Golgi complexes and extracellular accumulation of the hormone. The use of osmium greatly improved the fine structure of the tissue and its stability during acrylic embedment.

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Year:  1986        PMID： 3539083

Source DB:  PubMed          Journal:  Basic Appl Histochem        ISSN： 0391-7258


  4 in total

1.  Subcellular localisation of VEGF in different pituitary cells. Changes of its expression in oestrogen induced prolactinomas.

Authors:  Jorge Humberto Mukdsi; Ana Lucía De Paul; Silvina Gutiérrez; Félix Daniel Roth; Agustín Aoki; Alicia Inés Torres
Journal:  J Mol Histol       Date:  2006-05-19       Impact factor: 2.611

2.  Different behavior of lactotroph cell subpopulations in response to angiotensin II and thyrotrophin-releasing hormone.

Authors:  A De Paul; P Pons; A Aoki; A Torres
Journal:  Cell Mol Neurobiol       Date:  1997-04       Impact factor: 5.046

3.  Fate of degenerating lactotrophs in rat pituitary gland after interruption of lactation: a histochemical and immunocytochemical study.

Authors:  E M Orgnero de Gaisán; C A Maldonado; A Aoki
Journal:  Histochem J       Date:  1993-02

4.  Immunolocalization of Pit-1 in gonadotroph nuclei is indicative of the transdifferentiation of gonadotroph to lactotroph cells in prolactinomas induced by estrogen.

Authors:  Jorge Humberto Mukdsi; Ana Lucía De Paul; Sonia Muñoz; Agustín Aoki; Alicia Inés Torres
Journal:  Histochem Cell Biol       Date:  2004-06-09       Impact factor: 4.304
  4 in total

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