| Literature DB >> 35384636 |
Chenglu Wang1, Fangyu Wang2, Min Zheng1, Ruifang Wu1, Tianyun Yang3, Xiaopan Chen4,5, Jing Shu6,7.
Abstract
The aim of this study was to evaluate the effects of sperm proteasome activity on fertilization outcome and embryo development after IVF. Following density gradient centrifugation for IVF purpose, the spermatozoa of 84 infertile patients with tubal factor were evaluated by luciferase enzymatic activity to assess the proteasome quantity. The mean age of patients was 33.8 years, and the mean concentration of human spermatozoa 26S proteasome was 674.53 ng/ml. After IVF, the embryos were scored for morphology. The spermatozoa proteasome activity was both positively correlated with fertilization rate in vitro (P = 0.0003) and 2PN rate (P = 0.0007). Compared to low fertilization rate group, the high fertilization rate group showed a significantly higher level of spermatozoa proteasome activity (P = 0.002). In conclusion, sperm proteasome activity provides additional data on sperm functional capacity in terms of fertilization during IVF.Entities:
Keywords: 2-pronuclear formation rate; In vitro fertilization; Proteasome activity; Sperm parameter
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Year: 2022 PMID： 35384636 DOI： 10.1007/s43032-022-00916-z
Source DB: PubMed Journal: Reprod Sci ISSN： 1933-7191 Impact factor: 3.060