Kátia Cristina Silva Nascimento1, Sandra Maria de Oliveira Souza2, Aline Fagundes3, Roger Magno Macedo Silva4, Francisco Odencio Rodrigues de Oliveira Junior5, Suzana Corte-Real2, Juliana Helena da Silva Barros6. 1. Trypanosomatid Biology Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. 2. Structural Biology Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. 3. Clinical Research and Surveillance in Leishmaniasis Laboratory, Infectology National Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. 4. Rudolf Barth Electron Microscopy Platform, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. 5. Celular Ultrastructure Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. 6. Trypanosomatid Biology Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. juliana.barros@ioc.fiocruz.br.
Abstract
PURPOSE: Trypanosoma caninum exhibits atypical epimastigote forms under axenic conditions. This study aimed to analyze this evolutionary form under different cultivation conditions and provide more information about this evolutionary form. METHODS: We selected a T. caninum isolate with a high percentage of aflagellar epimastigote forms in axenic cultures. Two separate growth curves were generated for T. caninum cultured in Schneider axenic medium and co-cultured with the DH82 cell line, followed by analysis and quantification of evolutionary forms using bright field microscopy. In addition, ultrastructural analysis of T. caninum was performed under both cultivation conditions. RESULTS: The growth curves of T. caninum under axenic and co-cultivation conditions exhibited similar profiles. However, in the axenic culture, the number of parasites was three times higher at the peak of the exponential phase than in the co-culture. In contrast to that in the axenic culture, in which only the epimastigote forms were observed along the entire curve, during co-cultivation with the DH82 cell line, differentiation was observed for the trypomastigote and spheromastigote forms in low proportions. These results demonstrated that when cultured alone, the T. caninum isolate preserved the aflagellar epimastigote form, but in the presence of DH82 canine macrophages, they differentiated into evolutionary forms, particularly trypomastigote forms. Moreover, this study is the first to describe the presence of lipid bodies, structure described as the parasite's nutritional reserve, throughout the body of T. caninum. CONCLUSIONS: These findings describe biological and ultrastructural aspects of epimastigote aflagellar and suggest that this evolutionary form may be involved in the biological cycle of T. caninum, still unknown.
PURPOSE: Trypanosoma caninum exhibits atypical epimastigote forms under axenic conditions. This study aimed to analyze this evolutionary form under different cultivation conditions and provide more information about this evolutionary form. METHODS: We selected a T. caninum isolate with a high percentage of aflagellar epimastigote forms in axenic cultures. Two separate growth curves were generated for T. caninum cultured in Schneider axenic medium and co-cultured with the DH82 cell line, followed by analysis and quantification of evolutionary forms using bright field microscopy. In addition, ultrastructural analysis of T. caninum was performed under both cultivation conditions. RESULTS: The growth curves of T. caninum under axenic and co-cultivation conditions exhibited similar profiles. However, in the axenic culture, the number of parasites was three times higher at the peak of the exponential phase than in the co-culture. In contrast to that in the axenic culture, in which only the epimastigote forms were observed along the entire curve, during co-cultivation with the DH82 cell line, differentiation was observed for the trypomastigote and spheromastigote forms in low proportions. These results demonstrated that when cultured alone, the T. caninum isolate preserved the aflagellar epimastigote form, but in the presence of DH82 canine macrophages, they differentiated into evolutionary forms, particularly trypomastigote forms. Moreover, this study is the first to describe the presence of lipid bodies, structure described as the parasite's nutritional reserve, throughout the body of T. caninum. CONCLUSIONS: These findings describe biological and ultrastructural aspects of epimastigote aflagellar and suggest that this evolutionary form may be involved in the biological cycle of T. caninum, still unknown.
Authors: Tatiana S Fonseca-Oliveira; Juliana Helena S Barros; Juliana Bernardo Madeira; Raquel da Silva Pacheco; Carlos Roberto Alves; Luzia M C Côrtes; Mauro Célio de A Marzochi; Maria de Fatima Madeira Journal: Acta Trop Date: 2018-07-17 Impact factor: 3.112
Authors: O M M Thekisoe; T Honda; H Fujita; B Battsetseg; T Hatta; K Fujisaki; C Sugimoto; N Inoue Journal: Parasitology Date: 2007-02-19 Impact factor: 3.234
Authors: Maria F Madeira; Arleana B P F Almeida; Juliana H S Barros; Tatiana S F Oliveira; Valeria R F Sousa; Andreia S Alves; Luciana F C Miranda; Armando O Schubach; Mauro C A Marzochi Journal: J Parasitol Date: 2013-12-13 Impact factor: 1.276
Authors: Juliana H S Barros; Tatiana S Fonseca; Roger M Macedo-Silva; Suzana Côrte-Real; Helena K Toma; Maria de Fatima Madeira Journal: Acta Trop Date: 2014-05-29 Impact factor: 3.112