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Literature DB >> 3536804

Influence of embedding media in prolactin labelling with immunogold techniques.

Abstract

Immunogold labelling of prolactin in three different embedding media was compared. The polymeric prolactin in secretory granules was labelled in the three media, however, acrylic monomers (Lowicryl K4M and LR White) provided a more intense labelling with higher dilutions of the primary antibody, compared to the labelling in the epoxy resin (araldite). An intense labelling of monomeric prolactin in Golgi complex was detected only in acrylic embedments, and the labelling on the rough endoplasmic reticulum was significant only in LR White embedded tissues.

Entities: Chemical Gene

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Year: 1986 PMID： 3536804 DOI： 10.1007/BF01675335

Source DB: PubMed Journal: Histochem J ISSN： 0018-2214

8 in total

1. Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.

Authors: J Roth; M Bendayan; L Orci
Journal: J Histochem Cytochem Date: 1978-12 Impact factor: 2.479

2. Ultrastructural immunocytochemical localization of prolactin in rat anterior pituitary by use of the unlabeled antibody enzyme method.

Authors: J A Parsons; S L Erlandsen
Journal: J Histochem Cytochem Date: 1974-05 Impact factor: 2.479

3. An immunocolloid method for the electron microscope.

Authors: W P Faulk; G M Taylor
Journal: Immunochemistry Date: 1971-11

4. Classifications of anterior pituitary cell types with immunoenzyme histochemistry.

Authors: P K Nakane
Journal: J Histochem Cytochem Date: 1970-01 Impact factor: 2.479

5. Subcellular compartmentation of prolactin in rat lactotrophs.

Authors: A I Torres; A Aoki
Journal: J Endocrinol Date: 1985-05 Impact factor: 4.286

6. Immunolabeling of adenohypophysial cells with protein A-colloidal gold--antibody complex for electron microscopy: use of the freeze-substitution technique in tissue preparation.

Authors: S Hisano; T Adachi; S Daikoku
Journal: J Histochem Cytochem Date: 1984-07 Impact factor: 2.479

7. Isolation of two different pools of pituitary prolactin.

Authors: A I Torres; E S Haggi; A Aoki
Journal: Acta Endocrinol (Copenh) Date: 1984-09

8. Ultrastructural localization of prolactin in the rat anterior pituitary glands by preembedding peroxidase-labeled antibody method: observations in normal, castrated, or estrogen-stimulated specimen.

Authors: R Y Osamura; N Komatsu; S Izumi; S Yoshimura; K Watanabe
Journal: J Histochem Cytochem Date: 1982-09 Impact factor: 2.479

8 in total

7 in total

5. Fate of degenerating lactotrophs in rat pituitary gland after interruption of lactation: a histochemical and immunocytochemical study.

Authors: E M Orgnero de Gaisán; C A Maldonado; A Aoki
Journal: Histochem J Date: 1993-02

6. Cardiodilatin-immunoreactivity in specific atrial granules of human heart revealed by the immunogold stain.

Authors: C A Maldonado; W Saggau; W G Forssmann
Journal: Anat Embryol (Berl) Date: 1986

7. Renin and cathepsin B in human pituitary lactotroph cells. An ultrastructural study.

Authors: J P Saint-André; V Rohmer; F Pinet; M C Rousselet; J C Bigorgne; P Corvol
Journal: Histochemistry Date: 1989