Literature DB >> 35358284

Expression of Concern: SH2 Modified STAT1 Induces HLA-I Expression and Improves IFN-γ Signaling in IFN-α Resistant HCV Replicon Cells.

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Abstract

Entities:  

Year:  2022        PMID: 35358284      PMCID: PMC8970497          DOI: 10.1371/journal.pone.0266497

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


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After this article [1] was published, concerns were raised about western blots in Figs 2, 5 and 7, and microscopy images in Figs 1C, 6 and 8. Specifically: In Fig 1C in this article, the GR17-1 IFN-γ (-) panel appears similar to the 15–3 Cells IFN-α (+) panel in Fig 7a in [2], and the GR17-1 IFN-γ (+) panel appears similar to the IRF9 panel in Fig 7A in [2]. In Fig 2 in this article, the backgrounds of lanes one and two in the p-Jak2 blot appear similar to each other. In Fig 5 in this article, lanes three, four, six, and seven in the GR17-1 blot appear similar to each other. In Fig 6A in this article, the differential interference contrast image for IFN-γ (-) appears similar to the S9-13 IFN-γ (-) STAT1-CC-GFP panel in Fig 6B with 90° rotation. The differential interference contrast image in Fig 6A for IFN-γ (+) appears similar to the S9-13 IFN-γ (+) STAT1-GFP panel in Fig 6B with 90° rotation. In Fig 6B in this article, the GR17-1 IFN-γ (+) STAT1-GFP panel appears similar to the GR17-1 IFN-γ (-) STAT1-CC-GFP panel. In Fig 7 in this article, the GADPH blot appears similar to the HCV blot with an additional band in the final lane for the GADPH blot. In Fig 8 in this article, the S-Huh7 panel appears similar to the Huh-7 Cells IFN-α (-) panel in Fig 7A in [2] and to the Huh-7 Cells IFN-α (+) panel in Fig 7A in [2]. Follow-up on these issues is ongoing; in the meantime, the PLOS ONE Editors issue this Expression of Concern.
  2 in total

1.  SH2 modified STAT1 induces HLA-I expression and improves IFN-γ signaling in IFN-α resistant HCV replicon cells.

Authors:  Bret Poat; Sidhartha Hazari; Partha K Chandra; Feyza Gunduz; Luis A Balart; Xavier Alvarez; Srikanta Dash
Journal:  PLoS One       Date:  2010-09-30       Impact factor: 3.240

2.  Intracellular expression of IRF9 Stat fusion protein overcomes the defective Jak-Stat signaling and inhibits HCV RNA replication.

Authors:  Bret Poat; Sidhartha Hazari; Partha K Chandra; Feyza Gunduz; Xavier Alvarez; Luis A Balart; Robert F Garry; Srikanta Dash
Journal:  Virol J       Date:  2010-10-12       Impact factor: 4.099

  2 in total

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