Effects of decomposition on the recoverability of biological fluid evidence.

The present research assessed how the physical and chemical changes associated with decomposition affect the detection and identification of blood and semen evidence, as well as subsequent DNA analysis. A feeder pig (postmortem interval (PMI) < 3 h) was placed within the Boston University Outdoor Research Facility for a period of 22 days. Human blood and semen were individually dispensed onto multiple areas of two cotton t-shirts; one layer of fabric was placed above and below the pig and a control. One of each sample type was collected per day for a period of 22 days from each location. It was observed that both sample types when collected from beneath the pig exhibited the greatest decline in enzymatic activity over the course of testing, followed by samples from beneath the control, which can be inferred from the increase in negative screening results compared to the other samples. Spermatozoa were observed in nearly all semen samples, even when all screening results were negative, which lead to the generation of comparable DNA profiles for nearly all semen samples typed. Genetic typing of the blood samples beneath the pig and control rarely yielded comparable data while the samples from above yielded full profiles for all but a few samples tested.