| Literature DB >> 35352253 |
E K Karsuntseva1, G A Fursa2, A O Sosnovtseva1, A D Voronova3, A V Chadin1, A S Semkina1,4, O V Stepanova1,5, V P Chekhonin1,4.
Abstract
A gene-cell construct based on rat olfactory mucosa ensheathing cells transduced with an adenoviral vector encoding a mature form of brain neurotrophic factor (mBDNF) was transplanted into post-traumatic cysts of rat spinal cord. Transplantation of the gene-cell construct improved motor activity of the hind limbs and reduced the size of cysts in some animals. However, comparison of the effects of transduced and non-transduced ensheathing cells revealed no significant differences. In parallel in vitro experiments, a decrease in the proliferation of transduced cells compared to non-transduced cells was observed. It is likely that mBDNF reduces proliferation of transduced cells, which can affect their efficiency. The therapeutic efficacy of the new gene-cell construct is most likely provided by the cellular component.Entities:
Keywords: adenovector; cell therapy; gene therapy; olfactory mucosa ensheathing cells; spinal cord cysts
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Year: 2022 PMID： 35352253 DOI： 10.1007/s10517-022-05446-x
Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804