Literature DB >> 35349133

Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata.

Kamila Godel-Jędrychowska1, Tilman Franke2, Ewa Kurczyńska3.   

Abstract

Array tomography (AT) is a new high-throughput imaging method for high-resolution imaging of ultrastructure and for 3-D reconstruction of cells and organelles. Here, we describe the entire procedure for obtaining a spatial image of the distribution of plasmodesmata (PD). As example, the protocol is applied here to reconstruct the number and arrangement of PD between cells undergoing differentiation during Arabidopsis somatic embryogenesis.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  3-D reconstruction; Array tomography; Plasmodesmata; Ultrastructure

Mesh：

Year:  2022        PMID： 35349133     DOI： 10.1007/978-1-0716-2132-5_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  1 in total

1.  3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy.

Authors:  Matthew R G Russell; Thomas R Lerner; Jemima J Burden; David O Nkwe; Annegret Pelchen-Matthews; Marie-Charlotte Domart; Joanne Durgan; Anne Weston; Martin L Jones; Christopher J Peddie; Raffaella Carzaniga; Oliver Florey; Mark Marsh; Maximiliano G Gutierrez; Lucy M Collinson
Journal:  J Cell Sci       Date:  2016-07-21       Impact factor: 5.285
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.