| Literature DB >> 35347276 |
Ju-Hyun Lee1, Hyogeun Shin2, Mohammed R Shaker1, Hyun Jung Kim1, Si-Hyung Park1, June Hoan Kim1, Namwon Lee3, Minjin Kang4, Subin Cho5, Tae Hwan Kwak6, Jong Woon Kim7, Mi-Ryoung Song8, Seung-Hae Kwon9, Dong Wook Han6, Sanghyuk Lee5,10, Se-Young Choi11, Im Joo Rhyu1, Hyun Kim1, Dongho Geum4, Il-Joo Cho2,12, Woong Sun13.
Abstract
Human spinal-cord-like tissues induced from human pluripotent stem cells are typically insufficiently mature and do not mimic the morphological features of neurulation. Here, we report a three-dimensional culture system and protocol for the production of human spinal-cord-like organoids (hSCOs) recapitulating the neurulation-like tube-forming morphogenesis of the early spinal cord. The hSCOs exhibited neurulation-like tube-forming morphogenesis, cellular differentiation into the major types of spinal-cord neurons as well as glial cells, and mature synaptic functional activities, among other features of the development of the spinal cord. We used the hSCOs to screen for antiepileptic drugs that can cause neural-tube defects. hSCOs may also facilitate the study of the development of the human spinal cord and the modelling of diseases associated with neural-tube defects.Entities:
Mesh：
Year: 2022 PMID： 35347276 DOI： 10.1038/s41551-022-00868-4
Source DB: PubMed Journal: Nat Biomed Eng ISSN： 2157-846X Impact factor: 29.234