Comment on "Old is Gold: Electrolyte Aging Influences the Topography, Chemistry, and Bioactivity of Anodized TiO2 Nanopores".

Self-organized anodic TiO2 nanostructures, in the form of nanopores, nanotubes, mesosponge, etc., obtained by electrochemical anodization have in the past two decades attracted tremendous focus and the number of publications based on such structures for various applications is remarkable. Guo et al. in ACS Appl. Mater. Interfaces 2021, 13 (7), 7897-7912 discuss the obtaining of TiO2 nanopores by anodization in an aged electrolyte and its influence on the nanopores' topography, chemistry, and bioactivity. Guo et al. do not include in their study sufficient SEM characterization to confirm the claimed nanopore morphology. This comment clarifies the difference between a nanotube and a nanopore structure, both by already existing literature and reproduced experimental results. In fact, anodization on similar substrates and in similar anodization conditions leads to a nanotube morphology covered at the top by an initiation layer, depending on the anodization duration and post-treatments, such as ultrasonication. It is noted that the type of nanostructure has a clear influence on the available inner surface area for porosity computation or biomedical applications, for example, targeting drug delivery and interactions with proteins. Here, a clearer classification into nanopores and nanotubes─with an open top or an initiation layer─is established and explained.