Literature DB >> 3533724

Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli.

C N Chang, W J Kuang, E Y Chen.   

Abstract

The nucleotide sequence of the alkaline phosphatase (APase) gene (phoA) of Escherichia coli strain 294 has been determined. Pre-APase has a total of 471 amino acids (aa) including a signal sequence of 21 aa. The derived aa sequence differs from that obtained by protein sequencing by the presence of aspartic acid instead of asparagine at positions 16 and 36, and glutamic acid instead of glutamine at position 197. Two open reading frames (ORF1 and ORF2) located downstream from phoA or upstream from proC have been found. ORF1 encodes a putative presecretory protein of 106 aa with a signal sequence of 21 or 22 aa. If this protein is actually produced, it may be one of the smallest periplasmic proteins in E. coli.

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Year:  1986        PMID： 3533724     DOI： 10.1016/0378-1119(86)90050-8

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  48 in total

1.  Characterization of a two-component signal transduction system involved in the induction of alkaline phosphatase under phosphate-limiting conditions in Synechocystis sp. PCC 6803.

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Review 2.  Membrane topology and insertion of membrane proteins: search for topogenic signals.

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4.  TnphoA and TnphoA' elements for making and switching fusions for study of transcription, translation, and cell surface localization.

Authors:  M R Wilmes-Riesenberg; B L Wanner
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

5.  Regions of Rhodobacter sphaeroides cytochrome c2 required for export, heme attachment, and function.

Authors:  J P Brandner; E V Stabb; R Temme; T J Donohue
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

6.  Construction, expression, and localization of a CycA::PhoA fusion protein in Rhodobacter sphaeroides and Escherichia coli.

Authors:  A R Varga; S Kaplan
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

7.  Use of transposon TnphoA to identify genes for cell envelope proteins of Escherichia coli required for long-chain fatty acid transport: the periplasmic protein Tsp potentiates long-chain fatty acid transport.

Authors:  A Azizan; P N Black
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

8.  Contribution of regulation by the bvg locus to respiratory infection of mice by Bordetella pertussis.

Authors:  T J Merkel; S Stibitz; J M Keith; M Leef; R Shahin
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

9.  Lyme disease-causing Borrelia species encode multiple lipoproteins homologous to peptide-binding proteins of ABC-type transporters.

Authors:  J A Kornacki; D B Oliver
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

Review 10.  Strategies for achieving high-level expression of genes in Escherichia coli.

Authors:  S C Makrides
Journal:  Microbiol Rev       Date:  1996-09
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