Chunlei Wang1, Tao Zhang1, Lin Yang1, Xinyu Na1, Yanlong Qu2. 1. The Third Department of Orthopedic, The First Affiliate Hospital of Harbin Medical University, Youzheng Street, Nangang District, Harbin, 150001, Heilongjiang, China. 2. The Third Department of Orthopedic, The First Affiliate Hospital of Harbin Medical University, Youzheng Street, Nangang District, Harbin, 150001, Heilongjiang, China. quyanlong16@sina.com.
Abstract
BACKGROUND: Osteosarcoma (OS) is a rare malignant primary tumor of mesenchymal origin affecting bone that occurs in adolescents and children. LncRNAs are important regulators of tumorigenesis and development. This study aimed to explore the role and molecular basis of LncRNA BACE1-AS (BACE1 antisense RNA) in OS. METHODS AND RESULTS: Through the analysis of differential expressed lncRNAs in OS tissues by GEO database, LncRNA BACE1-AS display a remarkably lower expression. This found can also be observed in both OS tissues and cell lines by qRT-PCR. Furthermore, using Cell counting kit-8 (CCK-8), transwell, wound healing and westernblot assays, overexpression LncRNA BACE1-AS remarkably reduce cell proliferation, migration and invasion abilities in OS. In addition, LncRNA BACE1-AS is validated as a sponge of miR-762 through the prediction of lncRNASNP. Further, luciferase reporter and RIP assays are conducted to confirm the binding sites between LncRNA BACE1-AS and miR-762. SRY-box transcription factor 7 (SOX7) target to miR-762 and regulated by LncRNA BACE1-AS. Moreover, inhibition of miR-762 attenuate the role of sh-LncRNA BACE1-AS in OS cells, at meanwhile reduce the expression of SOX7. CONCLUSION: In this study, LncRNA BACE1-AS regulates proliferation, migration and invasion of osteosarcoma cells by miR-762/SOX7 axis, implying that LncRNA BACE1-AS is a potential target for osteosarcoma therapy.
BACKGROUND: Osteosarcoma (OS) is a rare malignant primary tumor of mesenchymal origin affecting bone that occurs in adolescents and children. LncRNAs are important regulators of tumorigenesis and development. This study aimed to explore the role and molecular basis of LncRNA BACE1-AS (BACE1 antisense RNA) in OS. METHODS AND RESULTS: Through the analysis of differential expressed lncRNAs in OS tissues by GEO database, LncRNA BACE1-AS display a remarkably lower expression. This found can also be observed in both OS tissues and cell lines by qRT-PCR. Furthermore, using Cell counting kit-8 (CCK-8), transwell, wound healing and westernblot assays, overexpression LncRNA BACE1-AS remarkably reduce cell proliferation, migration and invasion abilities in OS. In addition, LncRNA BACE1-AS is validated as a sponge of miR-762 through the prediction of lncRNASNP. Further, luciferase reporter and RIP assays are conducted to confirm the binding sites between LncRNA BACE1-AS and miR-762. SRY-box transcription factor 7 (SOX7) target to miR-762 and regulated by LncRNA BACE1-AS. Moreover, inhibition of miR-762 attenuate the role of sh-LncRNA BACE1-AS in OS cells, at meanwhile reduce the expression of SOX7. CONCLUSION: In this study, LncRNA BACE1-AS regulates proliferation, migration and invasion of osteosarcoma cells by miR-762/SOX7 axis, implying that LncRNA BACE1-AS is a potential target for osteosarcoma therapy.
Authors: Siobhan Simpson; Mark David Dunning; Simone de Brot; Llorenç Grau-Roma; Nigel Patrick Mongan; Catrin Sian Rutland Journal: Acta Vet Scand Date: 2017-10-24 Impact factor: 1.695