| Literature DB >> 35325429 |
Jinping Zhao1, Carlos Garcia Rios1, Jingjing Xu2, Ijaz Ahmad1, Junqi Song3,4.
Abstract
RNA interference (RNAi) is an evolutionarily conserved post-transcriptional gene silencing mechanism that responds to double-stranded RNA (dsRNA) by sequence-specific downregulation of target genes. The dsRNA-mediated RNAi technology has become one of the most widely used and powerful tools for functional genomic studies in diverse organisms. However, its application has been limited due to the technical difficulty of making RNAi constructs caused by the inverted repeat structure that is required for the formation of hairpin RNA. Here, we present a ligation-independent cloning-based dual vector-mediated RNAi system for silencing specific genes in plants. This approach is simple, efficient, and cost-effective and can be readily adapted to other binary vectors for functional analysis of target genes and the development of sustainable disease and pest control strategies in a broad range of plant species.Entities:
Keywords: Dual Vector System; Intron-Containing Hairpin RNA (ihpRNA); Ligation-Independent Cloning (LIC); Post-Transcriptional Gene Silencing (PTGS); RNA Interference (RNAi)
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Year: 2022 PMID： 35325429 DOI： 10.1007/978-1-0716-1875-2_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745