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Literature DB >> 35325427

Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control.

Tao Zhang¹, Fei Wang¹, Hui-Shan Guo^1,2, Yun Jin³.

Abstract

Trans-kingdom RNA interference (RNAi) has been reported in several plant-fungal pathosystems. Our recent works have demonstrated natural RNAi transmission from cotton plants into Verticillium dahliae, a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues, and successful application of trans-kingdom RNAi in cotton plants to confer Verticillium wilt disease resistance. Here, we provide a detailed protocol of cotton infection with V. dahliae, fungal hyphae recovery from infected cotton stems, and transmitted small RNA detection developed from our previous studies for trans-kingdom RNAi assays.

Entities: Chemical

Keywords: Cotton infection; Fungal hyphae recovery; Trans-kingdom small RNA; Verticillium dahliae

Mesh：

Year: 2022 PMID： 35325427 DOI： 10.1007/978-1-0716-1875-2_16

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control.

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