Literature DB >> 35325420

RTBV-Based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications.

Gaurav Kumar1, Kamlesh Kumari1, Indranil Dasgupta2.   

Abstract

The availability of protocols for virus-induced gene silencing (VIGS) in rice has opened up an important channel for the elucidation of gene functions in this important crop plant. Here, we present an updated protocol of a VIGS system based on Rice tungro bacilliform virus (RTBV) for gene silencing in rice. We present complete updated protocols for VIGS in rice, compare the system with other existing ones for monocots, identify some of the challenges faced by this system and discuss ways in which the vector could be improved for better silencing efficiency.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Agrobacterium tumefaciens; Agroinoculation; Monocot VIGS; Rice tungro bacilliform virus

Mesh：

Year:  2022        PMID： 35325420     DOI： 10.1007/978-1-0716-1875-2_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

Review 1.  RNA interference and small interfering RNAs.

Authors:  T Tuschl
Journal:  Chembiochem       Date:  2001-04-02       Impact factor: 3.164

2.  Virus-induced gene silencing in rice using a vector derived from a DNA virus.

Authors:  Arunima Purkayastha; Saloni Mathur; Vidhu Verma; Shweta Sharma; Indranil Dasgupta
Journal:  Planta       Date:  2010-09-25       Impact factor: 4.116

Review 3.  Virus-induced gene silencing (VIGS) in plants: an overview of target species and the virus-derived vector systems.

Authors:  Matthias Lange; Aravinda L Yellina; Svetlana Orashakova; Annette Becker
Journal:  Methods Mol Biol       Date:  2013

4.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

5.  Virus-induced gene silencing (VIGS) for functional genomics in rice using Rice tungro bacilliform virus (RTBV) as a vector.

Authors:  Ravi Kant; Shweta Sharma; Indranil Dasgupta
Journal:  Methods Mol Biol       Date:  2015

6.  A Positive Feedback Loop Governed by SUB1A1 Interaction with MITOGEN-ACTIVATED PROTEIN KINASE3 Imparts Submergence Tolerance in Rice.

Authors:  Pallavi Singh; Alok Krishna Sinha
Journal:  Plant Cell       Date:  2016-04-14       Impact factor: 11.277
  6 in total

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