Nayef Abdulrahman Louri1, Nigamananda Dey2,3, Rashed N AlHasan2, Safa Hassan Abdulla4, Mohamed Elsakka2, Rasheeqa Gulreez2, Abdulla Hassan Darwish4, Balamuthu Kadalmani3, Khalid Bin Ali Al Khalifa5. 1. Department of Plastic Surgery and Burn Unit, Bahrain Defence Force Royal Medical Services Military Hospital, Riffa, Southern Governorate, 28743, Bahrain. bdfburn@gmail.com. 2. Department of Plastic Surgery and Burn Unit, Bahrain Defence Force Royal Medical Services Military Hospital, Riffa, Southern Governorate, 28743, Bahrain. 3. Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India. 4. Department of Pathology, Bahrain Defence Force Royal Medical Services, Riffa, Bahrain. 5. Bariatric Metabolic Center, Bahrain Defence Force Royal Medical Services, Riffa, Bahrain.
Abstract
BACKGROUND: In extensive deep dermal burn injuries, split-thickness skin graft (STSG) has been the most preferred treatment option for resurfacing burn wounds. A thick split-thickness skin graft is ideal for preventing graft contracture but is associated with delayed donor healing and the lack of adequate donor skin. When applied with STSG, the dermal substitutes offer better-reconstructed skin than STSG alone. Human-derived acellular dermal matrix (HADM) obtained from cadaver skin is a dermal equivalent with good clinical outcomes. However, high cost and limited cadaver donor skin availability limit its clinical utility. Developing a low-cost preparation method and finding an alternate source of human donated skin can help reduce the cost. The objective of this study was to explore the feasibility of making HADM from abdominoplasty panniculus skin. METHODS: Skin samples were collected from the abdominoplasty panniculus of ten eligible donors with their informed consent. A combination of low-cost reagents-sodium chloride and hypotonic solution (water for injection) was used for decellularizing the skin. Characterization of the prepared Acellular Dermis Matrix prototype was done. RESULTS: The skin was deepidermized with one molar NaCl treatment at 37 °C for 24 h. The deepidermized dermis became acellular with hypotonic solution treatment at 4 °C for two weeks. The hematoxylin and eosin staining and cytotoxicity test confirmed the acellularity and non-cytotoxicity of the prepared HADM prototype. The HADM prototype also facilitated the formation of neo-epithelium in the 3D cell co-culture model. CONCLUSION: This study confirms that abdominoplasty panniculus can be a viable alternative for HADM preparation. Further characterization studies are required to prove the concept.
BACKGROUND: In extensive deep dermal burn injuries, split-thickness skin graft (STSG) has been the most preferred treatment option for resurfacing burn wounds. A thick split-thickness skin graft is ideal for preventing graft contracture but is associated with delayed donor healing and the lack of adequate donor skin. When applied with STSG, the dermal substitutes offer better-reconstructed skin than STSG alone. Human-derived acellular dermal matrix (HADM) obtained from cadaver skin is a dermal equivalent with good clinical outcomes. However, high cost and limited cadaver donor skin availability limit its clinical utility. Developing a low-cost preparation method and finding an alternate source of human donated skin can help reduce the cost. The objective of this study was to explore the feasibility of making HADM from abdominoplasty panniculus skin. METHODS: Skin samples were collected from the abdominoplasty panniculus of ten eligible donors with their informed consent. A combination of low-cost reagents-sodium chloride and hypotonic solution (water for injection) was used for decellularizing the skin. Characterization of the prepared Acellular Dermis Matrix prototype was done. RESULTS: The skin was deepidermized with one molar NaCl treatment at 37 °C for 24 h. The deepidermized dermis became acellular with hypotonic solution treatment at 4 °C for two weeks. The hematoxylin and eosin staining and cytotoxicity test confirmed the acellularity and non-cytotoxicity of the prepared HADM prototype. The HADM prototype also facilitated the formation of neo-epithelium in the 3D cell co-culture model. CONCLUSION: This study confirms that abdominoplasty panniculus can be a viable alternative for HADM preparation. Further characterization studies are required to prove the concept.
Authors: C D Richters; A Pirayesh; H Hoeksema; E W A Kamperdijk; R W Kreis; R P Dutrieux; S Monstrey; M J Hoekstra Journal: Cell Tissue Bank Date: 2008-05-20 Impact factor: 1.522
Authors: Sarah L White; Richard Hirth; Beatriz Mahíllo; Beatriz Domínguez-Gil; Francis L Delmonico; Luc Noel; Jeremy Chapman; Rafael Matesanz; Mar Carmona; Marina Alvarez; Jose R Núñez; Alan Leichtman Journal: Bull World Health Organ Date: 2014-08-22 Impact factor: 9.408