Tingting Fu1,2, Chuanzhi Li3, Zhongwei Sun4, Bing Yan4, Yanlin Wu5, Zhongxian Huang5, Xiao Yin6,7. 1. Department of Endocrinology and Metabolism, Jinan Central Hospital, Jinan, China, futing_ting@163.com. 2. Cheeloo College of Medicine, Shandong University, Jinan, China, futing_ting@163.com. 3. Department of Hepatobiliary Surgery, Jinan Central Hospital, Jinan, China. 4. Department of Gastrointestinal Surgery, Jinan Central Hospital, Jinan, China. 5. Department of Urinary Surgery, Jinan Central Hospital, Jinan, China. 6. Department of Endocrinology and Metabolism, Jinan Central Hospital, Jinan, China. 7. Cheeloo College of Medicine, Shandong University, Jinan, China.
Abstract
Introdution and Aims: The myokine irisin is critical to modulating adipocytes thermogenesis and influence whole-body metabolism. However, whether there is difference in the effects of irisin on adipocytes derived from different depots remains unknown, and the receptor of irisin on adipocytes is still unclear. In this study, we determine the browning effect of irisin on adipocytes of subcutaneous and visceral human adipose tissue and explore the possibility that integrin αV was the receptor of irisin on human adipocytes. METHODS: Human adipose-derived stem cells were isolated from human subcutaneous and visceral white adipose tissues and induced to differentiate into mature adipocytes, and the expression of UCP1 and thermogenic genes in mature adipocytes were examined with or without irisin treatment and compared between groups of different adiposity and different spots. Immunoprecipitation analysis was used to detect the interaction between irisin and integrin αV on adipocytes, and the protein expression of integrin αV in adipocytes was also compared between groups of different adiposity and anatomic position. RESULTS: Irisin treatment could increase the expression level of beige adipocyte marker protein UCP1 and specific thermogenic genes in mature adipocytes derived from subcutaneous white adipose tissue but not in visceral adipose tissue. The results of immunoprecipitation showed that irisin could be attached to integrin αV on mature adipocytes, and there was no significant difference in the gene and protein expression of integrin αV in adipocytes, either derived from subcutaneous and visceral adipose tissue, or derived from obese and normal-weight individuals. CONCLUSION: The results of the present study indicated that irisin contributed to the transformation of mature white adipocytes to beige adipocytes in human subcutaneous adipose tissue but not in visceral adipose tissue. Integrin αV may mediate the browning effects of irisin on human mature adipocytes, which could provide the potential therapeutic targets for obesity and metabolic syndrome by promoting human brown adipose tissue activity.
Introdution and Aims: The myokine irisin is critical to modulating adipocytes thermogenesis and influence whole-body metabolism. However, whether there is difference in the effects of irisin on adipocytes derived from different depots remains unknown, and the receptor of irisin on adipocytes is still unclear. In this study, we determine the browning effect of irisin on adipocytes of subcutaneous and visceral human adipose tissue and explore the possibility that integrin αV was the receptor of irisin on human adipocytes. METHODS: Human adipose-derived stem cells were isolated from human subcutaneous and visceral white adipose tissues and induced to differentiate into mature adipocytes, and the expression of UCP1 and thermogenic genes in mature adipocytes were examined with or without irisin treatment and compared between groups of different adiposity and different spots. Immunoprecipitation analysis was used to detect the interaction between irisin and integrin αV on adipocytes, and the protein expression of integrin αV in adipocytes was also compared between groups of different adiposity and anatomic position. RESULTS: Irisin treatment could increase the expression level of beige adipocyte marker protein UCP1 and specific thermogenic genes in mature adipocytes derived from subcutaneous white adipose tissue but not in visceral adipose tissue. The results of immunoprecipitation showed that irisin could be attached to integrin αV on mature adipocytes, and there was no significant difference in the gene and protein expression of integrin αV in adipocytes, either derived from subcutaneous and visceral adipose tissue, or derived from obese and normal-weight individuals. CONCLUSION: The results of the present study indicated that irisin contributed to the transformation of mature white adipocytes to beige adipocytes in human subcutaneous adipose tissue but not in visceral adipose tissue. Integrin αV may mediate the browning effects of irisin on human mature adipocytes, which could provide the potential therapeutic targets for obesity and metabolic syndrome by promoting human brown adipose tissue activity.
Authors: María Pardo; Ana B Crujeiras; María Amil; Zaida Aguera; Susana Jiménez-Murcia; Rosa Baños; Cristina Botella; Rafael de la Torre; Xavier Estivill; Ana B Fagundo; Jose M Fernández-Real; José C Fernández-García; Gema Fruhbeck; Javier Gómez-Ambrosi; Roser Rodríguez; Francisco J Tinahones; Fernando Fernández-Aranda; Felipe F Casanueva Journal: Int J Endocrinol Date: 2014-04-22 Impact factor: 3.257
Authors: Eben G Estell; Phuong T Le; Yosta Vegting; Hyeonwoo Kim; Christiane Wrann; Mary L Bouxsein; Kenichi Nagano; Roland Baron; Bruce M Spiegelman; Clifford J Rosen Journal: Elife Date: 2020-08-11 Impact factor: 8.140