Literature DB >> 35320561

Observing and Quantifying Fluorescent Reporters.

Sreeparna Pradhan1, Michael Hendricks2.   

Abstract

Genetically encoded fluorescent reporters take advantage of C. elegans' transparency to allow non-invasive, in vivo observation, and recording of physiological processes in intact animals. Here, we discuss the basic microscope components required to observe, image, and measure fluorescent proteins in live animals for students and researchers who work with C. elegans but have limited experience with fluorescence imaging and analysis.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Digital image analysis; Fluorescent proteins; Microscopy

Mesh：

Substances：

Year:  2022        PMID： 35320561     DOI： 10.1007/978-1-0716-2181-3_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  54 in total

1.  Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans.

Authors:  Jeffrey P Nguyen; Frederick B Shipley; Ashley N Linder; George S Plummer; Mochi Liu; Sagar U Setru; Joshua W Shaevitz; Andrew M Leifer
Journal:  Proc Natl Acad Sci U S A       Date:  2015-12-28       Impact factor: 11.205

2.  GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems.

Authors:  Evan H Feinberg; Miri K Vanhoven; Andres Bendesky; George Wang; Richard D Fetter; Kang Shen; Cornelia I Bargmann
Journal:  Neuron       Date:  2008-02-07       Impact factor: 17.173

3.  A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans.

Authors:  Shaohe Wang; Ngang Heok Tang; Pablo Lara-Gonzalez; Zhiling Zhao; Dhanya K Cheerambathur; Bram Prevo; Andrew D Chisholm; Arshad Desai; Karen Oegema
Journal:  Development       Date:  2017-06-15       Impact factor: 6.868

4.  daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans.

Authors:  S T Henderson; T E Johnson
Journal:  Curr Biol       Date:  2001-12-11       Impact factor: 10.834

5.  Green fluorescent protein as a marker for gene expression.

Authors:  M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
Journal:  Science       Date:  1994-02-11       Impact factor: 47.728

6.  Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans.

Authors:  R Kerr; V Lev-Ram; G Baird; P Vincent; R Y Tsien; W R Schafer
Journal:  Neuron       Date:  2000-06       Impact factor: 17.173

7.  Analysis of genetic mosaics of the nematode Caneorhabditis elegans.

Authors:  R K Herman
Journal:  Genetics       Date:  1984-09       Impact factor: 4.562

8.  NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification in C. elegans.

Authors:  Eviatar Yemini; Albert Lin; Amin Nejatbakhsh; Erdem Varol; Ruoxi Sun; Gonzalo E Mena; Aravinthan D T Samuel; Liam Paninski; Vivek Venkatachalam; Oliver Hobert
Journal:  Cell       Date:  2020-12-29       Impact factor: 41.582

9.  Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses.

Authors:  Siyu Feng; Aruna Varshney; Doris Coto Villa; Cyrus Modavi; John Kohler; Fatima Farah; Shuqin Zhou; Nebat Ali; Joachim D Müller; Miri K Van Hoven; Bo Huang
Journal:  Commun Biol       Date:  2019-09-17

10.  An expanded auxin-inducible degron toolkit for Caenorhabditis elegans.

Authors:  Guinevere E Ashley; Tam Duong; Max T Levenson; Michael A Q Martinez; Londen C Johnson; Jonathan D Hibshman; Hannah N Saeger; Nicholas J Palmisano; Ryan Doonan; Raquel Martinez-Mendez; Brittany R Davidson; Wan Zhang; James Matthew Ragle; Taylor N Medwig-Kinney; Sydney S Sirota; Bob Goldstein; David Q Matus; Daniel J Dickinson; David J Reiner; Jordan D Ward
Journal:  Genetics       Date:  2021-03-31       Impact factor: 4.562
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