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Literature DB >> 35320560

Genetic Methods for Cellular Manipulation in C. elegans.

Abstract

Neuron manipulation in vivo by ablation, activation or inactivation, and regulation of gene expression is essential for dissecting nervous system function. Here we describe genetic means for neuron manipulation in the nematode C. elegans, and provide protocols for generating transgenic animals containing these genetic tools.

Entities: Chemical

Keywords: C. elegans; Cell ablation; Microinjection; Neuronal activation; Neuronal silencing; Optogenetic; Transgenic worms

Mesh：

Year: 2022 PMID： 35320560 DOI： 10.1007/978-1-0716-2181-3_4

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

49 in total

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Authors: Christopher Fang-Yen; Christopher V Gabel; Aravinthan D T Samuel; Cornelia I Bargmann; Leon Avery
Journal: Methods Cell Biol Date: 2012 Impact factor: 1.441

2. Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG.

Authors: Yingchuan B Qi; Emma J Garren; Xiaokun Shu; Roger Y Tsien; Yishi Jin
Journal: Proc Natl Acad Sci U S A Date: 2012-04-24 Impact factor: 11.205

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Authors: Hiroshi Qadota; Makiko Inoue; Takao Hikita; Mathias Köppen; Jeffrey D Hardin; Mutsuki Amano; Donald G Moerman; Kozo Kaibuchi
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5. Rapid and permanent neuronal inactivation in vivo via subcellular generation of reactive oxygen with the use of KillerRed.

Authors: Daniel C Williams; Rachid El Bejjani; Paula Mugno Ramirez; Sean Coakley; Shin Ae Kim; Hyewon Lee; Quan Wen; Aravi Samuel; Hang Lu; Massimo A Hilliard; Marc Hammarlund
Journal: Cell Rep Date: 2013-10-31 Impact factor: 9.423

Genetic Methods for Cellular Manipulation in C. elegans.

Review 1. Laser microsurgery in Caenorhabditis elegans.

2. Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG.

3. Targeted cell killing by reconstituted caspases.

4. Establishment of a tissue-specific RNAi system in C. elegans.

5. Rapid and permanent neuronal inactivation in vivo via subcellular generation of reactive oxygen with the use of KillerRed.

6. Glia delimit shape changes of sensory neuron receptive endings in C. elegans.

7. A method for selective ablation of neurons in C. elegans using the phototoxic fluorescent protein, KillerRed.

Review 8. Adherens junctions in C. elegans embryonic morphogenesis.

9. Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination.

10. Efficient and cell specific knock-down of gene function in targeted C. elegans neurons.