Examining Galectin Gene Regulation by Reporter Assays.

Matching their role as potent and versatile effectors in cellular homeostasis and disease processes, galectins are subject to a fine-tuned transcriptional regulation of gene expression. It can apparently even involve coregulation with certain elements of the enzymatic machinery for glycan biosynthesis/remodeling and/or functional carriers of galectin-binding glycans such as the α5β1-integrin. All this suggests not yet fully known combinatorial processes to reach the desired outcome. Identification of transcription start point(s), cloning of upstream promoter region, and the design of plasmids for luciferase-based reporter assays establish the platform to initiate a systematic search of regulatory sequences. Their elucidation is also a step toward rationally manipulating expression of galectin genes in pathogenesis.