| Literature DB >> 35320523 |
Abstract
Surface plasmon resonance (SPR) instruments, like the BIAcore 3000, are useful for studying the binding between macromolecules in real time. The high sensitivity and low sample consumption in the Biacore enables the measurement of rapid kinetics and low affinities characteristics of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2 and -3 and their glycoside ligands using this approach.Entities:
Keywords: Affinity; Analyte; Flow cell (fc); Galectin; Glycosides; Ligand; Streptavidin (SA) sensor chip; Surface plasmon resonance (SPR)
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Year: 2022 PMID: 35320523 DOI: 10.1007/978-1-0716-2055-7_7
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745