Jnanranjan Panda1, Bhabani Sankar Satapathy2, Ratan Sarkar3, Bharati Tudu1. 1. Department of Physics, Jadavpur University, Kolkata, India. 2. School of Pharmaceutical Sciences, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, India. 3. Department of Physics, Jogesh Chandra Chaudhuri College, University of Calcutta, Kolkata, India.
Abstract
AIM: Docetaxel (DTX) loaded bio-compatible PLGA-PEG encapsulated zinc ferrite nanoparticles (ZFNP) formulation was developed and evaluated against C6 glioma cells. METHODS: The ZFNP were characterised using XRD, FE-SEM, TEM, etc. A series of drug formulations were fabricated by conjugating hydrothermally synthesised ZFNP with DTX in a PLGA-PEG matrix and optimised for drug loading. FTIR and DLS analysis of the formulation along with in vitro drug release, cytotoxicity, cellular uptake, and haemolytic effect were evaluated. RESULTS: Spherical, monodisperse, crystalline ZFNP with an average size of ∼28 nm were formed. The optimised formulation showed a hydrodynamic diameter of ∼147 nm, a surface charge of -34.8 mV, a drug loading of 6.9% (w/w) with prolonged drug release properties, and higher toxicity in C6 glioma cells compared to free DTX along with good internalisation and negligible haemolysis. CONCLUSION: The results indicate ZFNP could be effectively used as nanodrug carrier for delivery of docetaxel to glioma cells.
AIM: Docetaxel (DTX) loaded bio-compatible PLGA-PEG encapsulated zinc ferrite nanoparticles (ZFNP) formulation was developed and evaluated against C6 glioma cells. METHODS: The ZFNP were characterised using XRD, FE-SEM, TEM, etc. A series of drug formulations were fabricated by conjugating hydrothermally synthesised ZFNP with DTX in a PLGA-PEG matrix and optimised for drug loading. FTIR and DLS analysis of the formulation along with in vitro drug release, cytotoxicity, cellular uptake, and haemolytic effect were evaluated. RESULTS: Spherical, monodisperse, crystalline ZFNP with an average size of ∼28 nm were formed. The optimised formulation showed a hydrodynamic diameter of ∼147 nm, a surface charge of -34.8 mV, a drug loading of 6.9% (w/w) with prolonged drug release properties, and higher toxicity in C6 glioma cells compared to free DTX along with good internalisation and negligible haemolysis. CONCLUSION: The results indicate ZFNP could be effectively used as nanodrug carrier for delivery of docetaxel to glioma cells.