Literature DB >> 3531357

Serial cultivation of single keratinocytes from the outer root sheath of human scalp hair follicles.

A Limat, F K Noser.   

Abstract

A method for the isolation of outer root sheath keratinocytes from plucked human hair follicles and for their subsequent cultivation has been developed. The selective trypsinization of outer root sheath keratinocytes provided a single cell suspension of defined origin within the hair follicle. The 3T3 feeder layer technique supports sustained growth of these cells in that as little as one single plucked hair follicle (yielding approximately 1.5 X 10(4) cells) consistently gave rise to a confluent 35-mm culture dish (with approximately 1.5 X 10(6) cells) within about 2 weeks. The outer root sheath keratinocytes can be serially passaged for up to 3 times and also cryopreserved.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3531357     DOI： 10.1111/1523-1747.ep12455548

Source DB:  PubMed          Journal:  J Invest Dermatol        ISSN： 0022-202X            Impact factor:   8.551


  31 in total

1.  Characterization of a new tissue-engineered human skin equivalent with hair.

Authors:  M Michel; N L'Heureux; R Pouliot; W Xu; F A Auger; L Germain
Journal:  In Vitro Cell Dev Biol Anim       Date:  1999-06       Impact factor: 2.416

2.  [Treatment of recalcitrant wounds with autologous epidermal equivalents. After excision of multiple cylindromas of the scalp].

Authors:  A-K Tausche; G Richter-Huhn; G Sebastian
Journal:  Hautarzt       Date:  2004-03       Impact factor: 0.751

3.  Electron microscopic study of cultured cells from the murine hair tissues: cell growth and differentiation.

Authors:  N Tanigaki; H Ando; M Ito; A Hashimoto; Y Kitano
Journal:  Arch Dermatol Res       Date:  1990       Impact factor: 3.017

4.  Effects of testosterone, dihydrotestosterone and estradiol on growth of human hair outer root sheath keratinocytes in vitro.

Authors:  F Kiesewetter; A Arai; J Hintzenstern; H Schell
Journal:  Arch Dermatol Res       Date:  1991       Impact factor: 3.017

5.  Postmitotic human dermal fibroblasts preserve intact feeder properties for epithelial cell growth after long-term cryopreservation.

Authors:  A Limat; T Hunziker; C Boillat; F Noser; U Wiesmann
Journal:  In Vitro Cell Dev Biol       Date:  1990-07

6.  Interleukin 6 receptors on human outer root sheath cells and interfollicular epidermal keratinocytes in vitro: density-induced down regulation (DIDR) of receptors.

Authors:  E R Waelti; S P Inaebnit; U Weismann; A Limat; T Hunziker
Journal:  In Vitro Cell Dev Biol Anim       Date:  1996-05       Impact factor: 2.416

7.  Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells.

Authors:  Trond Aasen; Juan Carlos Izpisúa Belmonte
Journal:  Nat Protoc       Date:  2010-02-04       Impact factor: 13.491

8.  Differential epithelial outgrowth of plucked and microdissected human hair follicles in explant culture.

Authors:  I Moll
Journal:  Arch Dermatol Res       Date:  1996-09       Impact factor: 3.017

9.  Hair shaft elongation, follicle growth, and spontaneous regression in long-term, gelatin sponge-supported histoculture of human scalp skin.

Authors:  L Li; L B Margolis; R Paus; R M Hoffman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

10.  Outer root sheath (ORS) cells organize into epidermoid cyst-like spheroids when cultured inside Matrigel: a light-microscopic and immunohistological comparison between human ORS cells and interfollicular keratinocytes.

Authors:  A Limat; D Breitkreutz; T Hunziker; C E Klein; F Noser; N E Fusenig; L R Braathen
Journal:  Cell Tissue Res       Date:  1994-01       Impact factor: 5.249
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.