Literature DB >> 35311518

CRISPR Inhibition of Essential Peptidoglycan Biosynthesis Genes in Mycobacterium abscessus and Its Impact on β-Lactam Susceptibility.

Natalia Kurepina1, Liang Chen1,2, Kaelea Composto1, Dalin Rifat3, Eric L Nuermberger3, Barry N Kreiswirth1,2.   

Abstract

We utilized a CRISPR interference (CRISPRi) assay to control the gene expressions of two predicted essential peptidoglycan biosynthesis genes, pbpB and cwIM, in Mycobacterium abscessus and to evaluate their contribution to β-lactam susceptibility. Our results showed that CRISPR inhibition of each gene led to a significant 3-log10 reduction in CFU in the presence of imipenem but not for cefoxitin. These results demonstrate that CRISPRi provides an experimental approach to study drug/target interactions in M. abscessus.

Entities:  

Keywords:  CRISPRi; Mycobacterium abscessus; peptidoglycan biosynthesis; β-lactam

Mesh：

Substances：

Year:  2022        PMID： 35311518      PMCID： PMC9017322          DOI： 10.1128/aac.00093-22

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.938


  24 in total

1.  Non-tuberculous mycobacterial pulmonary disease.

Authors:  Steven Cowman; Jakko van Ingen; David E Griffith; Michael R Loebinger
Journal:  Eur Respir J       Date:  2019-07-11       Impact factor: 16.671

Review 2.  Mycobacterium abscessus Complex - a Particular Challenge in the Setting of Lung Transplantation.

Authors:  O Smibert; G I Snell; H Bills; G P Westall; C O Morrissey
Journal:  Expert Rev Anti Infect Ther       Date:  2016-02-03       Impact factor: 5.091

Review 3.  Advances in the management of pulmonary disease due to Mycobacterium abscessus complex.

Authors:  W-J Koh; J E Stout; W-W Yew
Journal:  Int J Tuberc Lung Dis       Date:  2014-10       Impact factor: 2.373

4.  Interaction between FtsW and penicillin-binding protein 3 (PBP3) directs PBP3 to mid-cell, controls cell septation and mediates the formation of a trimeric complex involving FtsZ, FtsW and PBP3 in mycobacteria.

Authors:  Pratik Datta; Arunava Dasgupta; Anil Kumar Singh; Partha Mukherjee; Manikuntala Kundu; Joyoti Basu
Journal:  Mol Microbiol       Date:  2006-12       Impact factor: 3.501

5.  Structures of Mycobacterium tuberculosis Penicillin-Binding Protein 3 in Complex with Five β-Lactam Antibiotics Reveal Mechanism of Inactivation.

Authors:  Zuokun Lu; Han Wang; Aili Zhang; Xiang Liu; Weihong Zhou; Cheng Yang; Luke Guddat; Haitao Yang; Christopher J Schofield; Zihe Rao
Journal:  Mol Pharmacol       Date:  2020-02-21       Impact factor: 4.436

6.  Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform.

Authors:  Jeremy M Rock; Forrest F Hopkins; Alejandro Chavez; Marieme Diallo; Michael R Chase; Elias R Gerrick; Justin R Pritchard; George M Church; Eric J Rubin; Christopher M Sassetti; Dirk Schnappinger; Sarah M Fortune
Journal:  Nat Microbiol       Date:  2017-02-06       Impact factor: 17.745

7.  Dual β-Lactam Combinations Highly Active against Mycobacterium abscessus Complex In Vitro.

Authors:  R Pandey; L Chen; C Manca; S Jenkins; L Glaser; C Vinnard; G Stone; J Lee; B Mathema; E L Nuermberger; R A Bonomo; B N Kreiswirth
Journal:  mBio       Date:  2019-02-12       Impact factor: 7.786

8.  Comparative genomics for mycobacterial peptidoglycan remodelling enzymes reveals extensive genetic multiplicity.

Authors:  Edith Erika Machowski; Sibusiso Senzani; Christopher Ealand; Bavesh Davandra Kana
Journal:  BMC Microbiol       Date:  2014-03-24       Impact factor: 3.605

9.  Genome-Wide Essentiality Analysis of Mycobacterium abscessus by Saturated Transposon Mutagenesis and Deep Sequencing.

Authors:  Dalin Rifat; Liang Chen; Barry N Kreiswirth; Eric L Nuermberger
Journal:  mBio       Date:  2021-06-15       Impact factor: 7.867

10.  Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosis.

Authors:  Barbara Bosch; Michael A DeJesus; Nicholas C Poulton; Wenzhu Zhang; Curtis A Engelhart; Anisha Zaveri; Sophie Lavalette; Nadine Ruecker; Carolina Trujillo; Joshua B Wallach; Shuqi Li; Sabine Ehrt; Brian T Chait; Dirk Schnappinger; Jeremy M Rock
Journal:  Cell       Date:  2021-07-22       Impact factor: 41.582
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  1 in total

1.  Strongly Bactericidal All-Oral β-Lactam Combinations for the Treatment of Mycobacterium abscessus Lung Disease.

Authors:  Dereje A Negatu; Matthew D Zimmerman; Véronique Dartois; Thomas Dick
Journal:  Antimicrob Agents Chemother       Date:  2022-09-01       Impact factor: 5.938
  1 in total

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