Literature DB >> 3530827

Preparation and characterization of a monoclonal antibody against boar acrosin.

J Pĕknicová, J Capková, D Cechová, B Sulcová.   

Abstract

BALB/c mice were immunized with the crude fraction of boar acrosin. Immune spleen cells were fused with myeloma cells SP2/0-Ag14. One of the 6 hybridomas produced was cloned and characterized by ELISA and SDS-PAGE. Possible uses of the monoclonal antibody against acrosin for immunological detection of the amount of acrosin liberated after manipulations with spermatozoa and for selection of undamaged spermatozoa for insemination are discussed.

Entities:  

Mesh:

Substances:

Year:  1986        PMID: 3530827

Source DB:  PubMed          Journal:  Folia Biol (Praha)        ISSN: 0015-5500            Impact factor:   0.906


  2 in total

1.  Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm.

Authors:  Hasmik Margaryan; Andriy Dorosh; Jana Capkova; Pavla Manaskova-Postlerova; Anatoly Philimonenko; Pavel Hozak; Jana Peknicova
Journal:  Reprod Biol Endocrinol       Date:  2015-03-08       Impact factor: 5.211

2.  Target antigens for Hs-14 monoclonal antibody and their various expression in normozoospermic and asthenozoospermic men.

Authors:  Jana Capkova; Hasmik Margaryan; Alena Kubatova; Petr Novak; Jana Peknicova
Journal:  Basic Clin Androl       Date:  2015-11-06
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.