Literature DB >> 35303481

"X" marks the spot: Mining the gold in CasX for gene editing.

Mitchell O Roth1, Hong Li2.   

Abstract

In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by ∼10-20 fold in vitro and mean-editing efficiency by ∼2-3 fold in human cells.
Copyright © 2022 Elsevier Inc. All rights reserved.

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Year:  2022        PMID： 35303481      PMCID： PMC9060429          DOI： 10.1016/j.molcel.2022.02.024

Source DB:  PubMed          Journal:  Mol Cell        ISSN： 1097-2765            Impact factor:   17.970


  9 in total

1.  Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.

Authors:  Bernd Zetsche; Jonathan S Gootenberg; Omar O Abudayyeh; Ian M Slaymaker; Kira S Makarova; Patrick Essletzbichler; Sara E Volz; Julia Joung; John van der Oost; Aviv Regev; Eugene V Koonin; Feng Zhang
Journal:  Cell       Date:  2015-09-25       Impact factor: 41.582

2.  Catalytically Enhanced Cas9 through Directed Protein Evolution.

Authors:  Travis H Hand; Mitchell O Roth; Chardasia L Smith; Emily Shiel; Kyle N Klein; David M Gilbert; Hong Li
Journal:  CRISPR J       Date:  2021-04

Review 3.  CRISPR-Cas9 Structures and Mechanisms.

Authors:  Fuguo Jiang; Jennifer A Doudna
Journal:  Annu Rev Biophys       Date:  2017-03-30       Impact factor: 12.981

4.  A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.

Authors:  Martin Jinek; Krzysztof Chylinski; Ines Fonfara; Michael Hauer; Jennifer A Doudna; Emmanuelle Charpentier
Journal:  Science       Date:  2012-06-28       Impact factor: 47.728

5.  Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity.

Authors:  Connor A Tsuchida; Shouyue Zhang; Mohammad Saffari Doost; Yuqian Zhao; Jia Wang; Elizabeth O'Brien; Huan Fang; Cheng-Ping Li; Danyuan Li; Zhuo-Yan Hai; Jonathan Chuck; Julian Brötzmann; Araz Vartoumian; David Burstein; Xiao-Wei Chen; Eva Nogales; Jennifer A Doudna; Jun-Jie Gogo Liu
Journal:  Mol Cell       Date:  2022-02-25       Impact factor: 19.328

6.  New CRISPR-Cas systems from uncultivated microbes.

Authors:  David Burstein; Lucas B Harrington; Steven C Strutt; Alexander J Probst; Karthik Anantharaman; Brian C Thomas; Jennifer A Doudna; Jillian F Banfield
Journal:  Nature       Date:  2016-12-22       Impact factor: 49.962

7.  Engineering of CRISPR-Cas12b for human genome editing.

Authors:  Jonathan Strecker; Sara Jones; Balwina Koopal; Jonathan Schmid-Burgk; Bernd Zetsche; Linyi Gao; Kira S Makarova; Eugene V Koonin; Feng Zhang
Journal:  Nat Commun       Date:  2019-01-22       Impact factor: 14.919

8.  CasX enzymes comprise a distinct family of RNA-guided genome editors.

Authors:  Jun-Jie Liu; Natalia Orlova; Benjamin L Oakes; Enbo Ma; Hannah B Spinner; Katherine L M Baney; Jonathan Chuck; Dan Tan; Gavin J Knott; Lucas B Harrington; Basem Al-Shayeb; Alexander Wagner; Julian Brötzmann; Brett T Staahl; Kian L Taylor; John Desmarais; Eva Nogales; Jennifer A Doudna
Journal:  Nature       Date:  2019-02-04       Impact factor: 49.962

Review 9.  Detect and destroy: CRISPR-based technologies for the response against viruses.

Authors:  Catherine A Freije; Pardis C Sabeti
Journal:  Cell Host Microbe       Date:  2021-04-28       Impact factor: 31.316
  9 in total

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