Literature DB >> 35294756

Cell-Based Assays to Identify Modulators of Nrf2/ARE Pathway.

Zhengxi Wei1, Jinghua Zhao1, Li Zhang1, Menghang Xia2.   

Abstract

The nuclear factor erythroid 2-related factor (Nrf2) and antioxidant response element (ARE) signaling pathway play an important role in the amelioration of cellular oxidative stress. Thus, assays that detect this pathway can be useful for identifying chemicals that induce or inhibit oxidative stress signaling. This chapter is to describe two cell-based Nrf2/ARE assays in a quantitative high-throughput screening (HTS) format to test a large collection of chemicals for oxidative stress induction ability. The assay descriptions involve cell handling, assay preparation, instrument usage, and assay procedure.
© 2022. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

Entities:  

Keywords:  Antioxidant response element (ARE); KeratinoSens; Luciferase (luc); Nuclear factor erythroid 2-related factor 2 (Nrf2); Reactive oxygen species (ROS); β-Lactamase (bla)

Mesh：

Substances：

Year:  2022        PMID： 35294756      PMCID： PMC9438791          DOI： 10.1007/978-1-0716-2213-1_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro.

Authors:  Roger Emter; Graham Ellis; Andreas Natsch
Journal:  Toxicol Appl Pharmacol       Date:  2010-03-20       Impact factor: 4.219

Review 2.  Cell survival responses to environmental stresses via the Keap1-Nrf2-ARE pathway.

Authors:  Thomas W Kensler; Nobunao Wakabayashi; Shyam Biswal
Journal:  Annu Rev Pharmacol Toxicol       Date:  2007       Impact factor: 13.820

Review 3.  The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress.

Authors:  Truyen Nguyen; Paul Nioi; Cecil B Pickett
Journal:  J Biol Chem       Date:  2009-01-30       Impact factor: 5.157

Review 4.  Nuclear factor-erythroid 2-related factor 2 as a chemopreventive target in colorectal cancer.

Authors:  Constance Lay Lay Saw; Ah-Ng Tony Kong
Journal:  Expert Opin Ther Targets       Date:  2011-01-25       Impact factor: 6.902

5.  Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter.

Authors:  G Zlokarnik; P A Negulescu; T E Knapp; L Mere; N Burres; L Feng; M Whitney; K Roemer; R Y Tsien
Journal:  Science       Date:  1998-01-02       Impact factor: 47.728

Review 6.  Regulation of NF-E2-related factor 2 signaling for cancer chemoprevention: antioxidant coupled with antiinflammatory.

Authors:  Rong Hu; Constance Lay-Lay Saw; Rong Yu; Ah-Ng Tony Kong
Journal:  Antioxid Redox Signal       Date:  2010-08-17       Impact factor: 8.401

7.  Nrf2 responses and the therapeutic selectivity of electrophilic compounds in chronic lymphocytic leukemia.

Authors:  Raymond P Wu; Tomoko Hayashi; Howard B Cottam; Guangyi Jin; Shiyin Yao; Christina C N Wu; Michael D Rosenbach; Maripat Corr; Richard B Schwab; Dennis A Carson
Journal:  Proc Natl Acad Sci U S A       Date:  2010-04-05       Impact factor: 11.205

8.  Cul3-mediated Nrf2 ubiquitination and antioxidant response element (ARE) activation are dependent on the partial molar volume at position 151 of Keap1.

Authors:  Aimee L Eggler; Evan Small; Mark Hannink; Andrew D Mesecar
Journal:  Biochem J       Date:  2009-07-29       Impact factor: 3.857

9.  Profiling environmental chemicals for activity in the antioxidant response element signaling pathway using a high throughput screening approach.

Authors:  Sunita J Shukla; Ruili Huang; Steven O Simmons; Raymond R Tice; Kristine L Witt; Danielle Vanleer; Ram Ramabhadran; Christopher P Austin; Menghang Xia
Journal:  Environ Health Perspect       Date:  2012-05-02       Impact factor: 9.031

10.  The Nrf2/ARE Pathway: A Promising Target to Counteract Mitochondrial Dysfunction in Parkinson's Disease.

Authors:  Kemal Ugur Tufekci; Ezgi Civi Bayin; Sermin Genc; Kursad Genc
Journal:  Parkinsons Dis       Date:  2011-02-22
  10 in total

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