Saurabh Kumar Agnihotri1,2,3, Balawant Kumar4,3, Ankita Jain2, Anjali Anjali4,5, Mahendra Pal Singh Negi4, Rekha Sachan5, Madan Lal Brahma Bhatt1, Raj Kamal Tripathi4,5, Monika Sachdev2,6. 1. Department of Radiotherapy, King George's Medical University, Lucknow 226 003, India. 2. Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow 226 031, India. 3. The first and the second authors contributed equally to this work. 4. Division of Toxicology & Experimental Medicine, CSIR-Central Drug Research Institute, Lucknow 226 031, India. 5. Department of Obstetrics & Gynaecology, King George's Medical University, Lucknow 226 003, India. 6. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India.
Abstract
Background: This study correlates the serum levels of sCD95 & TNF-α with a simple cell-based assay to evaluate the capacity of the serum sample to induce apoptosis in Jurkat cells. Interlinking of these parameters can be explored to design a minimum invasive diagnostic strategy for cervical cancer (CC). Methods: Sera samples were assessed to induce apoptosis in Jurkat cells through FACS. Serum levels of sCD95 and TNF-α were measured by ELISA. JNK phosphorylation was evaluated in sera incubated Jurkat cells. Data was scrutinized through statistical analysis. Results: Significantly higher serum levels of sCD95 and lower TNF-α levels were observed in CC patients; their sera samples inhibited induction of apoptosis in Jurkat cells through reduced JNK phosphorylation. Statistical analysis linked these three parameters for the early screening of CC. Conclusion: Distinct sera levels of sCD95 & TNF-α in CC patients showed an anti-apoptotic effect, which can be considered for early detection of CC.
Background: This study correlates the serum levels of sCD95 & TNF-α with a simple cell-based assay to evaluate the capacity of the serum sample to induce apoptosis in Jurkat cells. Interlinking of these parameters can be explored to design a minimum invasive diagnostic strategy for cervical cancer (CC). Methods: Sera samples were assessed to induce apoptosis in Jurkat cells through FACS. Serum levels of sCD95 and TNF-α were measured by ELISA. JNK phosphorylation was evaluated in sera incubated Jurkat cells. Data was scrutinized through statistical analysis. Results: Significantly higher serum levels of sCD95 and lower TNF-α levels were observed in CC patients; their sera samples inhibited induction of apoptosis in Jurkat cells through reduced JNK phosphorylation. Statistical analysis linked these three parameters for the early screening of CC. Conclusion: Distinct sera levels of sCD95 & TNF-α in CC patients showed an anti-apoptotic effect, which can be considered for early detection of CC.
Authors: J M Walboomers; M V Jacobs; M M Manos; F X Bosch; J A Kummer; K V Shah; P J Snijders; J Peto; C J Meijer; N Muñoz Journal: J Pathol Date: 1999-09 Impact factor: 7.996
Authors: S Jodo; S Kobayashi; N Kayagaki; N Ogura; Y Feng; Y Amasaki; A Fujisaku; M Azuma; H Yagita; K Okumura; T Koike Journal: Clin Exp Immunol Date: 1997-01 Impact factor: 4.330
Authors: Adriana Aguilar-Lemarroy; Jose E Romero-Ramos; Vicente Olimon-Andalon; Georgina Hernandez-Flores; Jose M Lerma-Diaz; Pablo C Ortiz-Lazareno; Gilberto Morgan-Villela; Susana Del Toro-Arreola; Alejandro Bravo-Cuellar; Luis F Jave-Suarez Journal: BMC Cancer Date: 2008-04-11 Impact factor: 4.430