Literature DB >> 3528752

Genetic studies on the beta subunit of Escherichia coli RNA polymerase. IX. The role of the carboxy-terminus in enzyme assembly.

R E Glass, A Honda, A Ishihama.   

Abstract

The assembly of RNA polymerase was studied in Escherichia coli mutants encoding large N-terminal amber fragments of the beta subunit. Whereas the removal of up to 20% of the carboxy-terminus does not prevent the formation of premature core enzyme, the amber fragments seem to interfere with holoenzyme production. These studies permit, therefore, the localization of a region on the beta polypeptide involved in sigma binding.

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Year:  1986        PMID： 3528752     DOI： 10.1007/bf00422075

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  15 in total

1.  Biosynthesis of RNA polymerase in Escherichia coli. III. Identification of intermediates in the assembly of RNA polymerase.

Authors:  K Ito; Y Iwakura; A Ishihama
Journal:  J Mol Biol       Date:  1975-08-05       Impact factor: 5.469

2.  Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors:  S W Kessler
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

Review 3.  Genetics of bacterial RNA polymerases.

Authors:  T Yura; A Ishihama
Journal:  Annu Rev Genet       Date:  1979       Impact factor: 16.830

4.  Subunits of RNA polymerase in function and structure. 7. Structure of premature core enzyme.

Authors:  A Ishihama; H Aiba; T Saitoh; S Takahashi
Journal:  Biochemistry       Date:  1979-03-20       Impact factor: 3.162

5.  Identification of an amber fragment of the beta subunit of Escherichia coli RNA polymerase: a yardstick for measuring controls on RNA polymerase subunit synthesis.

Authors:  R E Glass
Journal:  Mol Gen Genet       Date:  1977-02-28

6.  Subunits of RNA polymerase in function and structure. IV. Enhancing role of sigma in the subunit assembly of Escherichia coli RNA polymerase.

Authors:  A Ishihama; R Fukuda; K Ito
Journal:  J Mol Biol       Date:  1973-09-05       Impact factor: 5.469

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors:  J P Chamberlain
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

9.  Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VIII. Localisation of a region involved in promoter selectivity.

Authors:  R E Glass; S T Jones; V Nene; T Nomura; N Fujita; A Ishihama
Journal:  Mol Gen Genet       Date:  1986-06

10.  Genetic studies on the beta subunit of Escherichia coli RNA polymerase. I. The effect of known, single amino acid substitutions in an essential protein.

Authors:  V Nene; R E Glass
Journal:  Mol Gen Genet       Date:  1982
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  10 in total

1.  Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase.

Authors:  R Landick; A Colwell; J Stewart
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

2.  In vivo cloning of a carboxy-terminal rpoB allele which confers altered transcriptional properties.

Authors:  G C Rowland; P P Lim; R E Glass
Journal:  Folia Microbiol (Praha)       Date:  1995       Impact factor: 2.099

3.  Mapping the sigma70 subunit contact sites on Escherichia coli RNA polymerase with a sigma70-conjugated chemical protease.

Authors:  J T Owens; R Miyake; K Murakami; A J Chmura; N Fujita; A Ishihama; C F Meares
Journal:  Proc Natl Acad Sci U S A       Date:  1998-05-26       Impact factor: 11.205

4.  RNA polymerase II mutants defective in transcription of a subset of genes.

Authors:  C Scafe; M Nonet; R A Young
Journal:  Mol Cell Biol       Date:  1990-03       Impact factor: 4.272

5.  Binding of the sigma 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting.

Authors:  D P Greiner; K A Hughes; A H Gunasekera; C F Meares
Journal:  Proc Natl Acad Sci U S A       Date:  1996-01-09       Impact factor: 11.205

6.  Sigma S-dependent overexpression of ftsZ in an Escherichia coli K-12 rpoB mutant that is resistant to the division inhibitors DicB and DicF RNA.

Authors:  K Cam; A Cuzange; J P Bouché
Journal:  Mol Gen Genet       Date:  1995-07-28

7.  Hierarchy of the strength of Escherichia coli stringent control signals.

Authors:  R E Glass; S T Jones; T Nomura; A Ishihama
Journal:  Mol Gen Genet       Date:  1987-11

8.  Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VIII. Localisation of a region involved in promoter selectivity.

Authors:  R E Glass; S T Jones; V Nene; T Nomura; N Fujita; A Ishihama
Journal:  Mol Gen Genet       Date:  1986-06

9.  Expression of cloned rpoB gene of Escherichia coli: a genetic system for the isolation of dominant negative mutations and overproduction of defective beta subunit of RNA polymerase.

Authors:  J Y Lee; K Zalenskaya; Y K Shin; J D McKinney; J H Park; A Goldfarb
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

10.  Identifying a transcription factor interaction site on RNA polymerase II.

Authors:  A M Skantar; A L Greenleaf
Journal:  Gene Expr       Date:  1995
  10 in total

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