| Literature DB >> 3528297 |
L K Poulsen, H J Malling, I Søndergaard, B Weeke.
Abstract
An immunoradiometric assay for immunoglobulin E (IgE) using high adsorption polystyrene test tubes as the solid phase (the 'Maxisorp' assay) is described. The sensitivity of the method was found to be 50 pg IgE/ml and the within-assay reproducibility was 3-7%. The accuracy was estimated by means of a comparison between the Maxisorp assay and paper radioimmunosorbent test (PRIST) and a correlation coefficient of 0.98 (P less than 0.001) was obtained. We conclude that the Maxisorp assay is a fast and reliable assay for IgE determination in cord blood, cell culture supernatants and other highly diluted IgE preparations.Entities:
Mesh：
Substances：
Year: 1986 PMID： 3528297 DOI： 10.1016/0022-1759(86)90513-2
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303