Literature DB >> 3528295

Immunofluorescent screening of monoclonal antibodies to surface antigens of animal and plant cells bound to polycarbonate membranes.

T Hough, M B Singh, I J Smart, R B Knox.   

Abstract

We have developed an immunofluorescent screening method in which animal or plant cells are immobilized without fixation on polycarbonate (Nuclepore) membranes. The technique does not involve centrifugation, and virtually no cells are lost during processing. Direct microscopic analysis of antibody binding provides an extremely sensitive and reproducible screening system for hybridoma supernatants.

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Year:  1986        PMID： 3528295     DOI： 10.1016/0022-1759(86)90509-0

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  5 in total

1.  Gametes and Fertilization: Maize as a Model System for Experimental Embryogenesis in Flowering Plants.

Authors:  C. Dumas; H. L. Mogensen
Journal:  Plant Cell       Date:  1993-10       Impact factor: 11.277

2.  Isolation of Sperms from the Pollen Tubes of Flowering Plants during Fertilization.

Authors:  K R Shivanna; H Xu; P Taylor; R B Knox
Journal:  Plant Physiol       Date:  1988-07       Impact factor: 8.340

3.  Improvement of Longevity and Viability of Sperm Cells Isolated from Pollen of Zea mays L.

Authors:  G Zhang; C M Williams; M K Campenot; L E McGann; D D Cass
Journal:  Plant Physiol       Date:  1992-09       Impact factor: 8.340

4.  Antibodies to pollen exines.

Authors:  D Southworth; M B Singh; T Hough; I J Smart; P Taylor; R B Knox
Journal:  Planta       Date:  1988-12       Impact factor: 4.116

5.  Monoclonal antibodies identify common and differentiation-specific antigens on the plasma membrane of guard cells of Vicia faba L.

Authors:  G Key; E W Weiler
Journal:  Planta       Date:  1988-12       Impact factor: 4.116
  5 in total

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