Eosinophils and associated parameters in different types of skin diseases related to elevated eosinophil levels.

Background: Eosinophils, basophils, white blood cells (WBC), and immunoglobulin E (IgE) play major roles in the pathogenesis of atopic dermatitis (AD), bullous pemphigoid (BP), drug reaction with eosinophilia and systemic symptoms (DRESS), and hypereosinophilic syndrome (HES). This study aimed to describe these parameters in different skin diseases and provide further information concerning the underlying pathogenesis.
Methods: A cross-sectional study of blood test results, including WBC count, peripheral eosinophil count, peripheral basophil/WBC percentage, and IgE level, from 115 cases of AD, 75 cases of BP, 55 cases of DRESS, 119 cases of HES, and 621 healthy volunteers was performed in China. Data from before and after treatment and the population distribution of different diseases were compared and described.
Results: All participants showed increased peripheral eosinophil counts, eosinophil/WBC ratios, IgE levels, and decreased peripheral basophil counts, with variance among the different disease groups. Peripheral eosinophil counts in HES patients and IgE level in AD patients increased the most prominently. No significant correlation existed among eosinophils, basophils, and IgE. An obvious decrease in eosinophil count was demonstrated after treatment in all 4 diseases. Conclusions: Eosinophils, basophils, and IgE exert functions in diverse skin diseases, presenting altered peripheral blood test results. In some cases, these changes are demonstrated only in the skin and not in the blood. Compared with the other parameters considered in this study, eosinophils seemed to be a better biomarker for treatment effects, regardless of the disease type. 2022 Annals of Translational Medicine. All rights reserved.

Introduction

Eosinophils have been traditionally considered as beneficial against infection. The infiltration of eosinophils in inflammatory site revealed their ambiguous functions. A wide range of skin disorders are associated with eosinophil infiltration and the possibility of peripheral blood eosinophilia. Exogenous stimuli provoke innate or adaptive type 2 response, causing the group 2 innate lymphoid cells (ILC2) or T helper 2 (Th2) cells, respectively, to release type 2 cytokines such as IL-5 and IL-13. Recruited to the tissue and activated by these cytokines, eosinophils release toxic granule proteins (eosinophil peroxidase, eosinophil cationic protein, eosinophil-derived neurotoxin, and major basic protein) and various cytokines. Besides exerting functions in host defense especially against helminth, eosinophils can cause edema by producing leukotrienes, and induce blister formation and pruritus in diverse skin disorders (1,2). Chronic spontaneous urticaria has been considered closely related to mast cells, but not eosinophils, with recent study revealing activation of eosinophils in the affected lesions and improvement after treatments against IL-5 (3). Atopic dermatitis (AD) is closely associated with type 2 T helper cell immunity and elevated immunoglobulin E (IgE) levels, resulting in relapses of pruritis and eczema. Impaired epidermal function, skin microbiome dysregulation, and abnormalities of Th2 immunity interact with one another to accelerate the occurrence of AD. It is estimated that AD affected 20% of children and 10% of adults in high-income countries (4). Bullous pemphigoid (BP) is an autoimmune blistering skin disease in which the body forms antibodies against BP180 and/or BP230, which are important components of hemidesmosomes. Antibodies against BP180 and/or BP230 could ultimately result in dermal-epidermal splitting and the formation of tense blisters. It has also been shown that eosinophils play an important role in blister formation (5). A survey in France showed that the incidence of BP was 0.00217% per year (6). The onset of drug reaction with eosinophilia and systemic symptoms (DRESS) syndrome, also called drug-induced hypersensitivity syndrome, is usually associated with drug use and is characterized by systematic manifestations that include rashes, fever, lymphadenopathy, eosinophilia, and liver dysfunction (7). Although the pathophysiology of DRESS remains unclear, it is widely acknowledged to result from delayed type IV hypersensitivity reactions (8). A retrospective study in the US identified the number of potential DRESS syndrome cases as 2.18 per 100,000 patients from 1980 to 2016 (9). With an absolute elevated peripheral eosinophil count ≥1,500/µL and a related wide array of clinical manifestations, hypereosinophilic syndrome (HES) is a group of various diseases without specific pathogenesis (10,11). Although no exact data are available, the prevalence of HES is estimated to be between 0.000315% and 0.0063% (12).

Although all of the abovementioned skin disorders seem to present with eosinophilia, the importance of eosinophils in each disease remains unknown. There is a need for better parameters to use during the course of diagnosis, treatment, or follow-up. In this cross-sectional study, peripheral blood samples from Chinese patients with AD, BP, DRESS, and HES and healthy controls were collected. The white blood cell (WBC) count, peripheral eosinophil count, peripheral basophil/WBC percentage, and IgE level were compared and described. We present the following article in accordance with the STROBE reporting checklist (available at https://atm.amegroups.com/article/view/10.21037/atm-22-99/rc).

Methods

Study design and data source

In this cross-sectional study conducted from 1 January 2018 to 30 September 2020, blood test results, including the WBC count, peripheral eosinophil count, peripheral basophil/WBC percentage, and IgE level, were collected from patients diagnosed with the 4 diseases of AD, BP, DRESS, and HES (). The AD patients were diagnosed according to the criteria of Hanifin (13), and the BP patients were diagnosed by pathological biopsy. When the peripheral eosinophil count exceeded 1,500 and other diseases were excluded, patients were considered to have HES (14). The DRESS patients were selected based on the Japanese Consensus Group diagnostic criteria (15). The healthy controls comprised 621 randomly selected volunteers and were compared with 115 AD patients, 75 BP patients, 55 DRESS patients, and 119 HES patients. The sample size was decided by the number of cases in the area.

Table 1

Comparison of blood test results in several skin diseases

Parameters		AD				BP				DRESS				HES				Control
		25th	MD	75th		25th	MD	75th		25th	MD	75th		25th	MD	75th		25th	MD	75th
Age		20	25	37		57	68	76		35	51	68		57	65	71		29	32	38
WBC (×109/L)		6.19	7.45	9.14		6.90	8.20	11.19		6.95	10.49	14.56		7.03	8.32	10.43		4.95	5.89	6.91
EOS (×106/L)		374	748	1166		22	154	902		66	352	1122		880	1573	2387		71	112	192
EOS% (%)		4.9	10.71	15.98		0.27	2.02	13.74		0.86	3.91	10.14		12.71	19.83	28.39		1.30	1.90	3.30
BASO (×106/L)		1.850	3.020	5.200		0.800	2.150	3.300		1.170	2.695	4.215		0.000	2.845	5.902		19.270	28.600	39.905
BASO% (%)		0.20	0.40	0.70		0.10	0.20	0.40		0.10	0.30	0.48		0.00	0.30	0.78		0.30	0.50	0.70
IgE (IU/mL)		1276.8	2640.0	2856.0		470.4	1668.0	2784.0		45.8	307.2	999.6		233.8	643.2	2640.0		44.4	115.0	320.4

AD, atopic dermatitis; BP, bullous pemphigoid; DRESS, drug reaction with eosinophilia and systemic symptoms; HES, hypereosinophilic syndrome; WBC, peripheral white blood cell count; EOS, peripheral eosinophil count; EOS%, peripheral eosinophil/WBC percentage; BASO, peripheral basophil count; BASO%, peripheral basophil/WBC percentage; MD, median values; 25th, 25th percentiles; 75th, 75th percentiles.

The included patients demonstrated relatively severe symptoms and were admitted to Huashan Hospital. Most of the participants were treated with steroids, while those who showed little improvement began taking compound glycyrrhizin tablets. After 90% of the affected skin area had improved and the pruritis score was reduced to below 1, most of the participants were discharged. Data were collected at admission, 1 week after discharge, and 2 weeks after discharge.

Statistical analysis

Data were analyzed using the software SPSS 26.0 (IBM Corp., Chicago, IL, USA). In the Shapiro-Wilk test of normality, the null hypothesis was rejected for all parameters, divided by different diseases, when α=0.1 and could not be reverted by normality transformation. Therefore, all of the comparisons among diseases were performed by nonparametric Kruskal-Wallis tests with α=0.05. Missing data were excluded through pairwise deletion of cases.

The Spearman correlation coefficients were calculated for IgE eosinophils, IgE basophils, and eosinophil basophils. The distribution of ages and genders was described for different disease groups.

Ethical statement

All procedures performed in this study involving human participants were in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by the Medical Ethics Committee of Huashan Institutional Review Board, Fudan University (KY 2020-1135) and informed consent was taken from all the patients.

Results

Descriptive analysis by age and gender

Except for BP–HES, all other pairwise comparisons demonstrated significant differences in age (shown in ). All of the figures in this article were created by Prism 8 (GraphPad Software Inc., La Jolla, CA, USA).

Figure 1

Age and gender distribution. (A) Age distribution. The AD patients were relatively young, while the BP and HES patients were older. (B) Gender distribution. Only the HES group had a significantly larger proportion (α=0.05) of male patients. The median value is labeled as a dot, and the interquartile range is plotted as a thin line. AD, atopic dermatitis; BP, bullous pemphigoid; DRESS, drug reaction with eosinophilia and systemic symptoms; HES, hypereosinophilic syndrome.

The majority of our healthy controls were young people. To test the possible interference caused by the age of the controls, the data were divided by age into 5 groups from 0 to 100, with each group representing a span of 20 years. There was no difference in the distribution of age groups within each group, indicating that age had little impact on the results of this study.

Pearson’s chi-square test showed that only the HES group had a significantly larger proportion (α=0.05) of male participants ().

Parameters in different groups

WBC

The control group had a significantly lower peripheral WBC count than the other groups (P values all equal to 0), with no significant difference among the 4 disease groups ().

Figure 2

Blood test results. The comparison of each parameter was plotted. The median values and the interquartile ranges demonstrated relative differences of the 4 disease groups and the control group. (A) Peripheral WBC count. The WBC count of the control group was significantly lower than that of the disease groups, while there was no statistically significant difference among the 4 diseases. The P value of the pairwise comparison between the control group and any disease group equaled 0 and is labeled ****. (B) Peripheral eosinophil count. All disease groups except the BP group (P=0.834) had significantly higher peripheral eosinophil counts than the control group. Patients with HES and AD showed similarly elevated eosinophil counts that were significantly higher than those of the other groups. (C) The ratio of eosinophils to WBCs in peripheral blood. A comparison among groups showed results similar to those for the peripheral eosinophil count. (D) Peripheral basophil count. The control group had a significantly higher peripheral basophil count than the 4 disease groups; the significance is labeled as ****. No significant difference existed among the 4 disease groups. (E) The ratio of basophils to WBC in peripheral blood. Only the pairwise comparisons N–AD, AD–HES, HES–DRESS, and DRESS–BP were not significantly different. The ratios for each group can be presented from highest to lowest as N–AD–HES–DRESS–BP, and only the adjacent pairs had similar results (P value higher than 0.05). (F) Peripheral IgE level. The control group had significantly lower IgE levels than the other groups. Patients with DRESS had an IgE level that was statistically higher than that of the control group but statistically lower than that of the other 3 groups. AD, BP, and HES patients showed significantly elevated IgE levels, while AD patients showed the most notably elevated IgE levels. The median value is labeled as a dot, and the interquartile range is plotted as a thin line. AD, atopic dermatitis; BP, bullous pemphigoid; DRESS, drug reaction with eosinophilia and systemic symptoms; HES, hypereosinophilic syndrome.

Eosinophils

Both the peripheral eosinophil count and eosinophil/WBC ratio were compared among the groups. These 2 parameters showed almost the same tendency among groups, with slight differences. The control group had the lowest eosinophil count and eosinophil/WBC ratio, similar to the BP group. Participants with DRESS had significantly higher levels than the control group and similar levels to the BP group. In contrast, the HES and AD groups had significantly higher levels than the other 3 groups ().

Basophils

Both the peripheral basophil count and the basophil/WBC ratio were calculated. The control group had a significantly higher basophil count than the other groups, with no significant difference among the 4 disease groups ().

Although the 4 disease groups seemed similar in terms of both peripheral WBC and basophil counts, the basophil/WBC ratios were different. Only the pairwise comparisons N–AD, AD–HES, HES–DRESS, and DRESS–BP presented no significant difference. This finding, combined with the information from the box plot, indicated that the disease groups can be ordered from highest to lowest as N–AD–HES–DRESS–BP, with only the adjacent 2 groups showing similar values (P value higher than 0.05) ().

IgE

The control group had significantly lower IgE levels than the other groups. Patients with DRESS had an IgE level that was statistically higher than that of the control group but lower than that of the other 3 groups. Participants with AD, BP, and HES showed significantly elevated IgE levels, while AD patients showed the most prominently elevated IgE levels ().

Eosinophil/basophil ratio

Some data were missing due to the 0 peripheral basophil count; thus, only 99 AD patients, 67 BP patients, 43 DRESS patients, 80 HES patients, and 615 healthy controls were included. The eosinophil/basophil ratio of the control group differed significantly from that of the disease groups, and the distribution was somewhat limited ().

Figure 3

The peripheral eosinophil/basophil ratio. The control group had a significantly lower and narrowly distributed eosinophil/basophil ratio than the disease groups. The median value is labeled as a dot, and the interquartile range is plotted as a thin line. The p-value of the pairwise comparison between the control group and any disease group equaled 0 and is labeled ****. AD, atopic dermatitis; BP, bullous pemphigoid; DRESS, drug reaction with eosinophilia and systemic symptoms; HES, hypereosinophilic syndrome.

Correlations between parameters

The Spearman correlation coefficients of IgE–eosinophils, IgE–basophils, and eosinophils–basophils were calculated and compared. There was no correlation between IgE levels and basophils (α=0.05) in any group. Eosinophils and IgE were weakly correlated in only the BP and control groups. Furthermore, eosinophils and basophils were weakly correlated only in the HES and control groups.

Parameters and disease severity

By comparing the data collected before admission and after discharge, the impact of treatment effects and disease severity on the parameters could be roughly described.

Basophils and IgE

The basophil/WBC ratio and IgE levels were measured only twice, once before admission and once 1 week after discharge. There were no sufficient data collected in the BP, DRESS, and control groups to support the analysis. Using the related-samples Wilcoxon signed rank test, IgE and basophil/WBC ratio of 3 samples from the AD group, IgE of 4 samples from the HES group, and the basophil/WBC ratio of 5 samples from the HES group were calculated. The IgE level and basophil/WBC ratio did not change significantly after discharge.

Eosinophils and WBCs

Eosinophils and WBCs were measured 3 times: before admission, 1 week after discharge, and 2 weeks after discharge. There were 62 samples from the AD group, 61 from the BP group, 52 from the HES group, and 15 from the DRESS group at 1 week post discharge, but at 2 weeks post discharge, there were only 10 from the AD group, 3 from the BP group, 26 from the HES group, and 8 from the DRESS group. When all of the data collected from 3 repeated measurements were considered together, both eosinophils and WBCs count did not satisfy the requirements for spherical symmetry, and the multivariate tests demonstrated no significant difference, which may be the result of the limited sample size.

Furthermore, when only data from before admission and 1 week after discharge were considered, which resulted in a larger sample size, the related-samples Wilcoxon signed rank test showed a significant decrease in the peripheral eosinophil count in the disease groups. In this analysis, 132 out of 189 participants experienced a decrease in the peripheral eosinophil count after the alleviation of the disease, regardless of disease group. Nonetheless, the discharged patients still had significantly higher eosinophil counts than the healthy controls.

Discussion

Eosinophil, basophil, WBC, and IgE levels were compared across the 4 disease groups. All patients showed increased peripheral eosinophil counts, eosinophil/WBC ratios, and IgE levels, with variance among the different disease groups. Although the decrease in the peripheral basophil count in the disease groups was not in line with the critical role of basophils, it might be explained by the fact that basophils were strongly recruited but weakly stimulated to proliferate. Eosinophils were shown to be a more useful biomarker for the treatment effect than the other parameters considered in this study given their significant decrease after discharge regardless of the disease group.

The distribution of age and gender was also analyzed. The AD patients tended to be younger, the BP and HES patients tended to be older, and the DRESS patients were relatively evenly distributed. Only the HES group showed a predominance of males, which is consistent with previous studies (16).

Function of eosinophils

Our data indicated that patients with AD, HES, and DRESS had obviously increased levels of peripheral eosinophils, a finding that is consistent with previous studies illustrating the role of eosinophils in the pruritus and itching associated with various skin diseases (17). By releasing toxic granule proteins and producing leukotrienes, either directly regulating blood vessels or indirectly stimulating mast cells, eosinophils also contribute to tissue edema (2).

Among these skin diseases linked to increased eosinophil levels, the AD and HES groups had the highest levels of peripheral eosinophils, indicating that eosinophils have an important role in the pathogenesis of these diseases. As previously reported, a study using the MC903 mouse model showed that depletion of eosinophils obviously improved impaired skin barrier function and alleviated the symptoms of AD (18).

Eosinophils are also indispensable in the formation of blisters in BP patients. They are observed lining along dermal-epidermal junction, and locating in blisters. Although autoantibody IgE has been shown to mediate BP blister formation in a humanized IgE receptor mouse model, it fails to do so when eosinophils are deficient (19,20). Eosinophils, after activated by IL-5, could cause splitting of dermal-epidermal junction in the absence of BP antibodies (20), but clinical trial using anti-IL-5 antibody mepolizumab failed to ameliorate BP symptoms (21). However, our data showed no significant difference between the BP and control groups in terms of either peripheral eosinophil count or the eosinophil/WBC ratio. This contradictory result might be attributed to the rough balance of eosinophils proliferated and recruited out of the peripheral pool.

A previous cross-sectional study reported that BP patients with eosinophilia were significantly older than patients without eosinophilia (22), which does not concur with our data. The age distribution showed no significant difference in patients with and without eosinophilia. Ethnicity might account for this contradiction.

Peripheral eosinophilia had been a key feature of DRESS, and cutaneous eosinophil infiltration was rarely reported. Treatments against IL-5 proved effective, suggesting the elevated eosinophil level in DRESS primarily depends on IL-5 (2).

The IL-5 and IL-3 production from aberrant T cells could explain HES in part. Perivascular infiltration of eosinophils and lymphocytes could be found in those patients (2). Our results also discovered significant increased eosinophil level in HES patients.

For skin diseases associated with elevated peripheral eosinophils, anti-inflammatory approaches and therapies targeting eosinophils or related cytokines may be effective. Corticosteroids and calcineurin inhibitors such as tacrolimus could suppress the function of eosinophils as well as those cytokines stimulating them. Antibodies blocking the upstream or downstream pathways of eosinophil are also useful. Literature stated that antibodies against IL-5, CD52, IL-13, IL-31 and thymic stromal lymphopoietin could reduce eosinophil inflammation (23). Apart from these traditional therapies, new discoveries point out possible future directions. Eosinophils with defected autophagy functions are presented with increased effector functions, exaggerating tissue inflammatory (24). Therapies targeting autophagy regulatory pathways may also be promising.

Function of basophils

Basophils are also associated with pruritus (25). Evidence has demonstrated that basophils infiltrate skin lesions, especially in BP and AD (26), and that basophils may have a greater influence than effector cells given their ability to present antigens in allergen-induced T(H)2 responses in vitro and in vivo (27).

Despite the various important roles of basophils, the disease groups in this study did not demonstrate elevated peripheral basophils, which is consistent with a previous study indicating that the number of basophils is significantly elevated in the skin, but not in the blood, in AD (28). Unlike eosinophils, the involvement of basophils in these skin diseases was not accompanied by peripheral basophilia. Eosinophils and basophils may both be recruited to the affected skin area, but basophils are less strongly stimulated to proliferate in peripheral blood. Whether this theory applies to other skin diseases requires further investigations involving biopsies of skin lesions.

In AD patients, basophils secrete histamine and IgE when induced by antigens (29). By eliciting an interleukin-3-independent basophil response, Th2 cell-dependent immunity in AD patients could be enhanced (30). A recent study also found that FcεRIa is upregulated on basophils in AD-associated inflammation (31). These significant effects of basophils on AD might account for our finding that the AD group presented a higher basophil/WBC ratio than the DRESS and BP groups. Recent study suggests IL-37b could reduce the activation of basophils, and alleviate atopic dermatitis (32). More convincing evidence might be observed in skin biopsy results.

Although AD resembles BP in terms of both peripheral basophil and peripheral WBC counts, the AD patients had a higher basophil/WBC ratio than BP patients. This might indicate autoimmunity in BP, with higher levels of lymphocytes.

Function of IgE

By combining to the receptor FcεRI, IgE could induce the degranulation of mast cells and basophils, attracting leukocytes and amplifying the inflammatory process (33). All of the disease groups had significantly higher IgE levels than the control group. However, some healthy control samples presented elevated IgE levels, while some patients presented normal IgE levels, suggesting greater variation in IgE than in other parameters measured.

It has been reported that basophils activated via their high-affinity IgE receptor could prolong basophil-endothelial interactions, inducing the accumulation of eosinophils (34,35). The upstream role of IgE is consistent with our observation of elevated IgE levels in the disease groups.

Of the 4 disease groups, the AD group demonstrated the highest IgE level, indicating the indispensable role of IgE in AD. A study showed that AD patients treated with systemic CyA demonstrated improved symptoms along with a reduction in IgE autoreactivity (36).

The criterion of 200 U/mL IgE is usually used to discriminate between intrinsic and extrinsic AD (37). It has been reported that the classic extrinsic phenotype applies to 80% AD patients and is associated with a higher likelihood of elevated IgE levels, eosinophilia, a history of atopy, and filaggrin mutations. The other 20% of AD patients have normal IgE levels, late disease onset, preserved skin barrier function, and no history of atopy (37). In our data, only 8 patients had IgE levels lower than 200 U/mL, while the other 102 patients had IgE levels higher than 200 U/mL. No significant difference in eosinophils existed between the intrinsic and extrinsic AD patients in this study, which might be attributed to the relatively severe condition of inpatients.

As an autoimmune disease, BP also demonstrated elevated IgE levels similar to those of AD, including autoreactive IgE specific for BP180 and BP230. Skin biopsies of BP patients showed self-reactive IgE binding to mast cells and/or eosinophils (38).

Eosinophil/basophil ratio

Compared with healthy controls, all the patients showed lower peripheral basophil counts and higher eosinophil/basophil ratio. Although unable to effectively diagnose patients of certain disease, these 2 parameters could be used to exclude healthy controls. Those with decreased peripheral basophil counts and increased eosinophil/basophil ratio are more likely to have these 4 skin disorders. Combining both eosinophil and basophil, this ratio may have the potential to better describe different changes in eosinophils and basophils. Whether this parameter could be used for diagnosis requires further research.

Correlations

In the control group, weak rank correlations existed between both eosinophils–IgE and eosinophils–basophils. Similar weak rank correlations were also present between eosinophils–IgE in the BP group and between eosinophils–basophils in the HES group. The absence of this weak correlation in the other diseases indicated the discrete functions of IgE, eosinophils, and basophils. Basophil counts and IgE levels demonstrated no rank correlation in any group. As allergic reactions are always associated with the elevation of IgE, this result might provide clues about the other functions of eosinophils and basophils, in addition to effector cells, in allergic reactions.

AD, prominently resulted from type 2 inflammation, showed evidently increased IgE and eosinophil levels. IgE might contribute to eosinophil recruitment and activation through degranulation of mast cells or direct modulation of eosinophils (39). Although both eosinophil and IgE are crucial for this allergic disease, they demonstrate no correlation, indicating distinct regulatory pathways. BP, as a typical auto-immune disease, showed evidently increased IgE level, slightly increased eosinophil level, and weakly correlated eosinophils-IgE results. This might indicate that these eosinophils were majorly recruited and activated in synchronization with IgE, with little stimulation from other pathways. DRESS has been described as a drug reaction owing to type 4 hypersensitivity reaction, with slightly increased eosinophil and IgE levels, which might be secondary to the T cell functioning. For HES patients, apart from the significantly elevated eosinophil level, the IgE level showed conspicuous variation. Since the diagnosis of HES simply depends on peripheral eosinophil counts, it is likely that detailed subdivisions await discovery.

Eosinophils may act as biomarkers of treatment effects

Numerous biomarkers are available for evaluating the severity or activity of a range of skin diseases. However, to make this valuation easier and more widely applicable, our data suggest that the peripheral eosinophil count is a reflection of the treatment effects. After their discharge from the hospital, 132 patients showed significantly decreased peripheral eosinophil counts, 38 patients showed a minor increase, and 19 patients maintained the same result, regardless of the disease type. Such significant changes were observed only in eosinophils and not in other parameters. Therefore, it can be concluded that, compared with other parameters in this study, eosinophils are an effective biomarker for evaluating treatment outcomes, regardless of disease type.

According to the results of this cross-sectional study of AD, BP, DRESS, and HES patients, both eosinophils and basophils have indispensable roles in the pathogenesis of these diseases that in some cases are demonstrated only in the skin and not in the blood. Differences in the roles of eosinophils, basophils, and IgE could account for the weak correlation among these parameters and may indicate that eosinophils have more functions beyond evoking allergic reactions. Compared with other parameters in this study, eosinophils can serve as a better biomarker for determining treatment effects.

To our knowledge, this is the first study to compare eosinophil, basophil, and IgE levels across skin diseases, including AD, BP, HES, and DRESS. However, a limitation of this study is that skin biopsies were not applied. Peripheral results can only partly reveal the underlying pathogenesis and are not always parallel to biopsy results. Although some of these diseases are considered rare, the sample size could be further enlarged to avoid biases and confirm our findings. A 2-week follow-up was designed to examine the changes in results after discharge, but this duration is too short to present an accurate picture for chronic diseases. A longer follow-up might provide more information. As this study was conducted in China and only in-patients were recruited, patients of other ethnicities or with mild conditions may return different outcomes.

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