Marzieh Pazooki1, Shahin Bonakdar2, Behafarid Ghalandari3, Shiva Irani4. 1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2. National Cell Bank Department, Pasteur Institute of Iran, Tehran, Iran. 3. State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China. 4. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. s.irani@srbiau.ac.ir.
Abstract
BACKGROUND: The purpose of this research was to investigate the in vitro osteogenic induction of MG-63 cells using topography and collagen protein printed on polydimethyl siloxane (PDMS). METHODS: ALKALINE PHOSPHATASE (ALP) assay, calcium content, alizarin red staining, immunocytochemistry (ICC), and real-time polymerase chain reaction (PCR) were used to evaluate the osteo-differentiation of human adipose stem cells on the MG-63 cell pattern, MG-63 cells/collagen pattern, and collagen pattern. Also, the differentiated cell shape was studied by crystal violet staining and scanning electron microscopy (SEM). RESULTS: Our results showed that calcium content and ALP activity increased significantly on the MG-63 cells /collagen pattern (P < 0.05). The gene expression analysis (ALKALINPHOSPHATASE, COLLAGEN1 and OSTEOCALCIN) and bone marker protein expression (OSTEOCALCIN) confirmed the osteo differentiation of adipose stem cells (ADSCs) seeded on the imprinting substrate. DISCUSSION: Cell and molecular printing enhanced osteogenic development of adipose stem cells, according to our findings.
BACKGROUND: The purpose of this research was to investigate the in vitro osteogenic induction of MG-63 cells using topography and collagen protein printed on polydimethyl siloxane (PDMS). METHODS: ALKALINE PHOSPHATASE (ALP) assay, calcium content, alizarin red staining, immunocytochemistry (ICC), and real-time polymerase chain reaction (PCR) were used to evaluate the osteo-differentiation of human adipose stem cells on the MG-63 cell pattern, MG-63 cells/collagen pattern, and collagen pattern. Also, the differentiated cell shape was studied by crystal violet staining and scanning electron microscopy (SEM). RESULTS: Our results showed that calcium content and ALP activity increased significantly on the MG-63 cells /collagen pattern (P < 0.05). The gene expression analysis (ALKALINPHOSPHATASE, COLLAGEN1 and OSTEOCALCIN) and bone marker protein expression (OSTEOCALCIN) confirmed the osteo differentiation of adipose stem cells (ADSCs) seeded on the imprinting substrate. DISCUSSION: Cell and molecular printing enhanced osteogenic development of adipose stem cells, according to our findings.
Authors: Jessica H Wen; Ludovic G Vincent; Alexander Fuhrmann; Yu Suk Choi; Kolin C Hribar; Hermes Taylor-Weiner; Shaochen Chen; Adam J Engler Journal: Nat Mater Date: 2014-08-10 Impact factor: 43.841