Ruoting Lin1, Shuang Zhou2, Huanan Zhao3, Huasong Lin1, Lixing Wang2, Weipeng Hu4, Hongzhi Gao2,4, Bin Qiu3. 1. Department of Neurology, Second Affiliated Hospital of Fujian Medical University Quanzhou 362000, Fujian, China. 2. Clinical Center of Molecular Diagnosis and Therapy, Second Affiliated Hospital of Fujian Medical University Quanzhou 362000, Fujian, China. 3. Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University Fuzhou 350108, Fujian, China. 4. Department of Neurosurgery, Second Affiliated Hospital of Fujian Medical University Quanzhou 362000, Fujian, China.
Abstract
OBJECTIVE: To propose a novel signal enhancement strategy based on the synergy between β-CD-CuNCs and multi-walled carbon nanotubes (MWCNTs) for the detection of DNA oxidative damage biomarker 8-Hydroxy-2'-deoxyguanosine (8-OHdG). METHODS: The sensor was constructed with the β-CD-CuNCs-MWCNTs-nafion film and successfully used for the quantitative detection of 8-OhdG in the presence of biomolecules such as ascorbic acid (AA) and uric acid (UA). To investigate the surface morphology of the modified electrode, Transmission Electron Microscopy (TEM), Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were performed on bare and modified electrodes. RESULTS: According to Differential Pulse Voltammetry (DPV) results, there was a linear relationship between peak current and concentration of 8-OhdG, ranging from 1.0×10-7 to 1.0×10-6 mol/L (R2=0.9926) and 1.0×10-6 to 2.0×10-5 mol/L (R2=0.9933). The detection limit (S/N=3) was 33 nmol/L. CONCLUSIONS: The proposed sensor had been successfully applied to the determination of 8-OHdG in human urine samples with high recovery rates. AJTR
OBJECTIVE: To propose a novel signal enhancement strategy based on the synergy between β-CD-CuNCs and multi-walled carbon nanotubes (MWCNTs) for the detection of DNA oxidative damage biomarker 8-Hydroxy-2'-deoxyguanosine (8-OHdG). METHODS: The sensor was constructed with the β-CD-CuNCs-MWCNTs-nafion film and successfully used for the quantitative detection of 8-OhdG in the presence of biomolecules such as ascorbic acid (AA) and uric acid (UA). To investigate the surface morphology of the modified electrode, Transmission Electron Microscopy (TEM), Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were performed on bare and modified electrodes. RESULTS: According to Differential Pulse Voltammetry (DPV) results, there was a linear relationship between peak current and concentration of 8-OhdG, ranging from 1.0×10-7 to 1.0×10-6 mol/L (R2=0.9926) and 1.0×10-6 to 2.0×10-5 mol/L (R2=0.9933). The detection limit (S/N=3) was 33 nmol/L. CONCLUSIONS: The proposed sensor had been successfully applied to the determination of 8-OHdG in human urine samples with high recovery rates. AJTR
Authors: Etiane Tatsch; Guilherme V Bochi; Sílvia J Piva; José A M De Carvalho; Helena Kober; Vanessa D Torbitz; Thiago Duarte; Cristiane Signor; Adriane C Coelho; Marta M M F Duarte; Greice F F S Montagner; Ivana B M Da Cruz; Rafael N Moresco Journal: Mutat Res Date: 2012-01-24 Impact factor: 2.433