Bing Yu1, Jinghua Pang1, Jiawen You1. 1. Department of Thoracic Surgery, Ningbo Fenghua District People's Hospital Ningbo 315500, Zhejiang Province, China.
Abstract
OBJECTIVES: To study the role of miR-133a expression in the invasion, proliferation, migration, and apoptosis of lung cancer cells and its mechanism. METHODS: miR-133a expression levels in human normal lung epithelial cells (BEAS-2B), H441 cell lines and NSCLC tissues were detected by qPCR. The influence of miR-133a mimics on the migration, proliferation and invasion of H441 cells was examined by CCK-8 assay, transwell migration assay, and invasion assay, respectively. Expression of MMP-9 and LASP1 in H441 cellstreated by miR-133a mimics was determined by western blot. Pearson's test was conducted to study the association of miR-133a expression with clinical characteristics of NSCLC patients. The targeted regulation of miR-133a on LASP1 gene expression was detected by the luciferase reporter gene assay. RESULTS: miR-133a expression was decreased in H441 cells in contrast to that in BEAS-2B cells (P<0.05). Compared with para-carcinoma tissues, miR-133a levels were markedly down-regulated in NSCLC tissues. miR-133a overexpression inhibited the invasion, proliferation, and migration ability of H441 cells and promoted cell apoptosis (all P<0.05). MMP-9 expression levels were also reduced in the miR-133a mimic group. Moreover, miR-133a expression levels were correlated with tumor size and TNM stage. miR-133a overexpression decreased the expression of LASP1, which is the targeted gene of miR-133a. CONCLUSIONS: miR-133a overexpression can reduce the invasion, proliferation, migration, and matrix metalloproteinase expression of NSCLC cells and promote cell apoptosis. This may be correlated to targeted down-regulation of LASP1 expression. AJTR
OBJECTIVES: To study the role of miR-133a expression in the invasion, proliferation, migration, and apoptosis of lung cancer cells and its mechanism. METHODS: miR-133a expression levels in human normal lung epithelial cells (BEAS-2B), H441 cell lines and NSCLC tissues were detected by qPCR. The influence of miR-133a mimics on the migration, proliferation and invasion of H441 cells was examined by CCK-8 assay, transwell migration assay, and invasion assay, respectively. Expression of MMP-9 and LASP1 in H441 cellstreated by miR-133a mimics was determined by western blot. Pearson's test was conducted to study the association of miR-133a expression with clinical characteristics of NSCLC patients. The targeted regulation of miR-133a on LASP1 gene expression was detected by the luciferase reporter gene assay. RESULTS: miR-133a expression was decreased in H441 cells in contrast to that in BEAS-2B cells (P<0.05). Compared with para-carcinoma tissues, miR-133a levels were markedly down-regulated in NSCLC tissues. miR-133a overexpression inhibited the invasion, proliferation, and migration ability of H441 cells and promoted cell apoptosis (all P<0.05). MMP-9 expression levels were also reduced in the miR-133a mimic group. Moreover, miR-133a expression levels were correlated with tumor size and TNM stage. miR-133a overexpression decreased the expression of LASP1, which is the targeted gene of miR-133a. CONCLUSIONS: miR-133a overexpression can reduce the invasion, proliferation, migration, and matrix metalloproteinase expression of NSCLC cells and promote cell apoptosis. This may be correlated to targeted down-regulation of LASP1 expression. AJTR
Authors: Lisha Ying; Lingbin Du; Ruiyang Zou; Lei Shi; Nan Zhang; Jiaoyue Jin; Chenyang Xu; Fanrong Zhang; Chen Zhu; Junzhou Wu; Kaiyan Chen; Minran Huang; Yingxue Wu; Yimin Zhang; Weihui Zheng; Xiaodan Pan; Baofu Chen; Aifen Lin; John Kit Chung Tam; Rob Martinus van Dam; David Tien Min Lai; Kee Seng Chia; Lihan Zhou; Heng-Phon Too; Herbert Yu; Weimin Mao; Dan Su Journal: Proc Natl Acad Sci U S A Date: 2020-09-17 Impact factor: 11.205
Authors: Johannes F Fahrmann; Dmitry Grapov; Brett S Phinney; Carol Stroble; Brian C DeFelice; William Rom; David R Gandara; Yanhong Zhang; Oliver Fiehn; Harvey Pass; Suzanne Miyamoto Journal: Clin Proteomics Date: 2016-10-27 Impact factor: 3.988
Authors: Sonia Blanco-Prieto; Leticia Barcia-Castro; María Páez de la Cadena; Francisco Javier Rodríguez-Berrocal; Lorena Vázquez-Iglesias; María Isabel Botana-Rial; Alberto Fernández-Villar; Loretta De Chiara Journal: BMC Cancer Date: 2017-12-05 Impact factor: 4.430