Literature DB >> 3527293

DNA cytosine methylation and heat-induced deamination.

M Ehrlich, K F Norris, R Y Wang, K C Kuo, C W Gehrke.   

Abstract

The heat-induced conversion of 5-methylcytosine (m5C) residues to thymine residues and of cytosine to uracil residues in single-stranded DNA was studied. The calculated rates for deamination at 37 degrees C and pH 7.4 were approximately 9.5 X 10(-10) and 2.1 X 10(-10) sec-1, respectively. N4-Methyldeoxycytidine, which is in the DNA of certain thermophilic bacteria, was more heat-resistant than was deoxycytidine and much more than was 5-methyldeoxycytidine. Thermophilic bacteria which contain N4-methylcytosine rather than m5C in their genomes may thereby largely avoid heat-induced mutation due to deamination, which is incurred by the many organisms that contain m5C in their DNA.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3527293     DOI： 10.1007/bf01116426

Source DB:  PubMed          Journal:  Biosci Rep        ISSN： 0144-8463            Impact factor:   3.840


  59 in total

1.  Modification of DNA duplexes to smooth their thermal stability independently of their base content for DNA sequencing by hybridization.

Authors:  H K Nguyen; P Auffray; U Asseline; D Dupret; N T Thuong
Journal:  Nucleic Acids Res       Date:  1997-08-01       Impact factor: 16.971

2.  Transfection of heteroduplexes containing uracil.guanine or thymine.guanine mispairs into plant cells.

Authors:  N M Inamdar; X Y Zhang; C L Brough; W E Gardiner; D M Bisaro; M Ehrlich
Journal:  Plant Mol Biol       Date:  1992-10       Impact factor: 4.076

3.  Sequence, internal homology and high-level expression of the gene for a DNA-(cytosine N4)-methyltransferase, M.Pvu II.

Authors:  T Tao; J Walter; K J Brennan; M M Cotterman; R M Blumenthal
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

4.  Is there an acceleration of the CpG transition rate during the mammalian radiation?

Authors:  M Peifer; J E Karro; H H von Grünberg
Journal:  Bioinformatics       Date:  2008-07-28       Impact factor: 6.937

5.  The stability of the RNA bases: implications for the origin of life.

Authors:  M Levy; S L Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1998-07-07       Impact factor: 11.205

6.  APOBEC3B is an enzymatic source of mutation in breast cancer.

Authors:  Michael B Burns; Lela Lackey; Michael A Carpenter; Anurag Rathore; Allison M Land; Brandon Leonard; Eric W Refsland; Delshanee Kotandeniya; Natalia Tretyakova; Jason B Nikas; Douglas Yee; Nuri A Temiz; Duncan E Donohue; Rebecca M McDougle; William L Brown; Emily K Law; Reuben S Harris
Journal:  Nature       Date:  2013-02-06       Impact factor: 49.962

7.  Cytosine deamination is a major cause of baseline noise in next-generation sequencing.

Authors:  Guoli Chen; Stacy Mosier; Christopher D Gocke; Ming-Tseh Lin; James R Eshleman
Journal:  Mol Diagn Ther       Date:  2014-10       Impact factor: 4.074

8.  HhaI and HpaII DNA methyltransferases bind DNA mismatches, methylate uracil and block DNA repair.

Authors:  A S Yang; J C Shen; J M Zingg; S Mi; P A Jones
Journal:  Nucleic Acids Res       Date:  1995-04-25       Impact factor: 16.971

9.  Cytosine deaminations catalyzed by DNA cytosine methyltransferases are unlikely to be the major cause of mutational hot spots at sites of cytosine methylation in Escherichia coli.

Authors:  M Wyszynski; S Gabbara; A S Bhagwat
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-15       Impact factor: 11.205

10.  Cytosine methylation by the SuaI restriction-modification system: implications for genetic fidelity in a hyperthermophilic archaeon.

Authors:  Dennis W Grogan
Journal:  J Bacteriol       Date:  2003-08       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.