Literature DB >> 35266828

Impact of Short-Term Storage on Ex Vivo Antimalarial Susceptibilities of Fresh Ugandan Plasmodium falciparum Isolates.

Martin Okitwi1, Stephen Orena1, Katairo Thomas1, Patrick K Tumwebaze1, Oswald Byaruhanga1, Samuel L Nsobya1, Melissa D Conrad2, Brett R Bayles3, Philip J Rosenthal2, Roland A Cooper3.   

Abstract

We measured susceptibilities of Ugandan Plasmodium falciparum isolates assayed on the day of collection or after storage at 4°C. Samples were incubated with serial dilutions of 8 antimalarials, and susceptibilities were determined from 72-h growth inhibition assays. Storage was associated with decreased growth and lower 50% inhibitory concentration values, but differences between assays beginning on day 0 or after 1 or 2 days of storage were modest, indicating that short-term storage before drug susceptibility determination is feasible.

Entities:  

Keywords:  Plasmodium falciparum; Uganda; antimalarials; cold storage; drug susceptibility; ex vivo; growth; malaria

Mesh：

Substances：

Year:  2022        PMID： 35266828      PMCID： PMC9017344          DOI： 10.1128/aac.01437-21

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.938


  11 in total

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