Literature DB >> 352589

Immunofluorescence titres of sperm centrifuged at different speeds.

R Kay, N J Alexander.   

Abstract

Two washing procedures were used on spermatozoa to test the effect of centrifugation on immunofluorescent staining. The rapidly centrifuged spermatozoa often exhibited a loss of acrosomal but an increase in tail antigenicity. A standard technique of sperm preparation for immunofluorescence is recommended in order to have reliable and comparable results.

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Year:  1978        PMID： 352589      PMCID： PMC1541286     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  6 in total

1.  Methods for the detection of antibodies against mammalian spermatozoa. II. A gelatin agglutination test.

Authors:  S KIBRICK; D L BELDING; B MERRILL
Journal:  Fertil Steril       Date:  1952 Sep-Oct       Impact factor: 7.329

2.  Antigenicity of frozen and fresh spermatozoa.

Authors:  N J Alexander; R Kay
Journal:  Fertil Steril       Date:  1977-11       Impact factor: 7.329

3.  Surface structure of spermatozoa frozen for artificial insemination.

Authors:  N J Alexander
Journal:  Andrologia       Date:  1977 Apr-Jun       Impact factor: 2.775

4.  The effects of washing on the ultrastructure and cytochemistry of ram spermatozoa.

Authors:  R C Jones; W V Holt
Journal:  J Reprod Fertil       Date:  1974-11

5.  Vasectomy: long-term effects in the rhesus monkey.

Authors:  N J Alexander
Journal:  J Reprod Fertil       Date:  1972-12

6.  Immunofluorescent studies on human spermatozoa. I. The detection of different spermatozoal antibodies and their occurrence in normal and infertile women.

Authors:  T Hjort; K B Hansen
Journal:  Clin Exp Immunol       Date:  1971-01       Impact factor: 4.330
  6 in total

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