Isolation and Transfection of Maize Endosperm Protoplasts.

Endosperm of cereal crops is the main component of its grain. Improvement of endosperm traits will bolster grain yield and quality. Functional analysis of endosperm trait-related genes often requires the use of an endosperm cell system. Here, we describe a protocol for the isolation and transfection of maize endosperm cell protoplast. The endosperm protoplast system can be used for several molecular studies including protein subcellular localization, protein-protein interaction by bimolecular fluorescence complementation (BiFC), protein immunoblotting, transient gene expression, and regulatory analyses by qRT-PCR.