Early responses in Penaeus vannamei during experimental infection with Enterocytozoon hepatopenaei (EHP) spores by injection and oral routes.

Hepatopancreatic microsporidiosis caused by Enterocytozoon hepatopenaei (EHP) is associated with severe production losses in Penaeus vannamei farming. Early responses in P. vannamei experimentally infected with EHP was assessed in this study by feeding infected hepatopancreatic tissue and by injecting purified EHP spores (∼1 × 105 Spores/shrimp). Immune responses to EHP infection were assessed in the haemolymph by analysing the total haemocyte count (THC), superoxide dismutase (SOD) activity, prophenoloxidase activity (proPO), respiratory burst activity (RBA), catalase activity (CAT), lysozyme activity (LYS) and Toll gene expression in hepatopancreas at 0, 6, 12, 24, 36, 48, 60 and 72 h post-infection (hpi). Experimental infection with EHP resulted in a significant (p < 0.05) reduction in the immune parameters such THC, CAT and LYS at 6, 24 and 24 hpi respectively while there was a significant increase (p < 0.05) in the levels of SOD, proPO and RBA at 6 hpi. The expression of the Toll gene was significantly upregulated (p < 0.05) after experimental infection with EHP from 6 hpi. These findings on immune responses in P. vannamei during EHP infection will assist in the development of suitable management measures to reduce the negative impacts of EHP in P. vannamei farming. This is the first report on early responses in P. vannamei during EHP infection.