Mohamad Tarik1, Lakshmy Ramakrishnan2, Nidhi Bhatia3, Ravindra Goswami4, Devasenathipathy Kandasamy5, Atanu Roy6, Dinu S Chandran6, Archna Singh7, Ashish Datt Upadhyay8, Mani Kalaivani8, Jayanthi Neelamraju9, Ratna Sudha Madempudi9, Reena Rajan3. 1. Department of Cardiac Biochemistry, All India Institute of Medical Sciences, New Delhi, 110029, India. 2. Department of Cardiac Biochemistry, All India Institute of Medical Sciences, New Delhi, 110029, India. lakshmy_ram@yahoo.com. 3. Department of Orthopaedics, All India Institute of Medical Sciences, New Delhi, 110029, India. 4. Department of Endocrinology & Metabolism, All India Institute of Medical Sciences, New Delhi, 110029, India. 5. Department of Radiodiagnosis, All India Institute of Medical Sciences, New Delhi, 110029, India. 6. Department of Physiology, All India Institute of Medical Sciences, New Delhi, 110029, India. 7. Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 110029, India. 8. Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, 110029, India. 9. Centre for Research & Development, Unique Biotech Ltd., Alexandria Knowledge Park, Hyderabad, 500038, Telangana, India.
Abstract
PURPOSE: The aim of the present study was to assess the effect of Bacillus coagulans Unique IS-2 supplementation on absorption and utilization of protein in resistance-trained males. METHODS: In this double blind, placebo-control trial, resistance-trained males (21.08 ± 2.84 years) were randomized to consume, either 20 g of whey protein powder {80% whey protein concentrate (WPC80), amounting to 15.4 g protein} with 2 billion CFU Bacillus coagulans Unique IS-2 (supplemental group) or 20 g of whey protein powder and lactose instead of Bacillus coagulans (placebo group) once daily for 60 days with a controlled resistance exercise protocol. The whey protein concentrate (WPC-80) given to both groups had a lactose content of 6.8%. Plasma-free amino acids (PFAAs) were determined at baseline, at 30 and 60 days of supplementation. Muscle strength, hypertrophy, VO2 max, and body composition, and other biochemical parameters were assessed at baseline and end line. RESULTS: A positive effect of probiotic Bacillus coagulans Unique IS-2 supplementation was observed on protein absorption as evidenced by an increase in total PFAA by + 16.1% (p = 0.004). Branched chain amino acids (BCAA) comprising isoleucine (p = 0.016), leucine (p = 0.001), and valine (p = 0.002) were increased by + 33.1% in ITT analysis as compared to placebo after 60 days. At 30 days an increase in isoleucine by + 35% (p = 0.113), leucine by + 43% (p = 0.032), and valine by + 32% (p = 0.017) was observed in ITT analysis. Probiotic effect was shown on exercise performance as evidenced by an increase in one RM of leg press and vertical jump power by + 16.61% (p = 0.024) and + 7.86% (p = 0.007), respectively. CONCLUSION: Significantly increased absorption of BCAA with supplementation of B. coagulans Unique IS-2 along with whey protein and improvement in leg press and vertical jump power was noted indicating the positive effect of the probiotic on muscle power in the lower body. TRIAL REGISTRATION NUMBER: CTRI/2017/03/008117; Date:16.03.2017.
PURPOSE: The aim of the present study was to assess the effect of Bacillus coagulans Unique IS-2 supplementation on absorption and utilization of protein in resistance-trained males. METHODS: In this double blind, placebo-control trial, resistance-trained males (21.08 ± 2.84 years) were randomized to consume, either 20 g of whey protein powder {80% whey protein concentrate (WPC80), amounting to 15.4 g protein} with 2 billion CFU Bacillus coagulans Unique IS-2 (supplemental group) or 20 g of whey protein powder and lactose instead of Bacillus coagulans (placebo group) once daily for 60 days with a controlled resistance exercise protocol. The whey protein concentrate (WPC-80) given to both groups had a lactose content of 6.8%. Plasma-free amino acids (PFAAs) were determined at baseline, at 30 and 60 days of supplementation. Muscle strength, hypertrophy, VO2 max, and body composition, and other biochemical parameters were assessed at baseline and end line. RESULTS: A positive effect of probiotic Bacillus coagulans Unique IS-2 supplementation was observed on protein absorption as evidenced by an increase in total PFAA by + 16.1% (p = 0.004). Branched chain amino acids (BCAA) comprising isoleucine (p = 0.016), leucine (p = 0.001), and valine (p = 0.002) were increased by + 33.1% in ITT analysis as compared to placebo after 60 days. At 30 days an increase in isoleucine by + 35% (p = 0.113), leucine by + 43% (p = 0.032), and valine by + 32% (p = 0.017) was observed in ITT analysis. Probiotic effect was shown on exercise performance as evidenced by an increase in one RM of leg press and vertical jump power by + 16.61% (p = 0.024) and + 7.86% (p = 0.007), respectively. CONCLUSION: Significantly increased absorption of BCAA with supplementation of B. coagulans Unique IS-2 along with whey protein and improvement in leg press and vertical jump power was noted indicating the positive effect of the probiotic on muscle power in the lower body. TRIAL REGISTRATION NUMBER: CTRI/2017/03/008117; Date:16.03.2017.
Authors: Paul O Sheridan; Laure B Bindels; Delphine M Saulnier; Gregor Reid; Esther Nova; Kerstin Holmgren; Paul W O'Toole; James Bunn; Nathalie Delzenne; Karen P Scott Journal: Gut Microbes Date: 2013-12-16
Authors: Tícia Kocsis; Bálint Molnár; Dávid Németh; Péter Hegyi; Zsolt Szakács; Alexandra Bálint; András Garami; Alexandra Soós; Katalin Márta; Margit Solymár Journal: Sci Rep Date: 2020-07-16 Impact factor: 4.379