| Literature DB >> 35248130 |
Ryan P Cheng1, Puneet Dang1, Alemji A Taku1, Yoon Ji Moon1, Vi Pham1, Xiaohe Sun1, Ethan Zhao1, Jonathan A Raper2.
Abstract
Entities:
Year: 2022 PMID: 35248130 PMCID: PMC8898399 DOI: 10.1186/s13064-022-00160-w
Source DB: PubMed Journal: Neural Dev ISSN: 1749-8104 Impact factor: 3.842
Fig. 6Misprojecting growth cones fail to retract in nrp2a and in sema3fa mutants. A Live imaging sequences of nrp2a heterozygote and mutant siblings, showing misprojecting axons occupying the dorsal-medial OB. The yellow dotted lines indicate the dorsal boundary of the developing DZ and CZ protoglomeruli and denote the edge of the dorsal-medial OB region. Yellow arrows indicate misprojecting axons. B The cumulative time that the dorsal-medial OB is occupied by TRPC2-class OSNs is greater in nrp2a mutants as compared to nrp2a heterozygous siblings. C The maximum distance TRPC2-class axons project into the dorsal-medial OB is greater in nrp2a mutants as compared to heterozygotes. D Live imaging sequences of sema3fa heterozygote and mutant siblings, showing misprojecting axons occupying the dorsal-medial OB. E The cumulative time that the dorsal-medial OB is occupied by TRPC2-class OSNs is greater in sema3fa mutants as compared to heterozygous siblings. F The maximum distance TRPC2-class axons project into the dorsal-medial OB is greater in sema3fa mutants as compared to heterozygotes. G Model of average TRPC2-class axon locations in sema3fa heterozygotes and mutants during live imaging sequence. TRPC2-class axons are shown in green and magenta, and OMP-class axons in grey. The three TRPC2 surfaces encompass axon location probabilities, from most transparent to most opaque, of 5.6, 18.7, and 31.8%. Yellow dotted line represents the edge of the dorsal-medial OB region
Fig. 5Both nrp2a and nrp2b act in the same pathway with sema3fa A Representative confocal sections of sema3fa mutant and nrp2a;sema3fa double mutant siblings. Yellow arrows indicate misprojecting axons. B The misprojection phenotype in nrp2a;sema3fa double mutants is not significantly different from sema3fa mutant siblings. C Representative confocal sections of sema3fa mutant and nrp2b;sema3fa double mutant siblings. Yellow arrows indicate misprojecting axons. D The misprojection phenotype in nrp2b;sema3fa double mutants is not significantly different from sema3fa mutant siblings. Model of average TRPC2-class axon locations in sema3fa heterozygotes and mutants during live imaging sequence. TRPC2-class axons are shown in green and magenta, and OMP-class axons in grey. The three TRCP2 surfaces encompass axon location probabilities, from most transparent to most opaque, of 5.6, 18.7, and 31.8%. Yellow dotted line represents the edge of the dorsal-medial OB region