Literature DB >> 3524512

Use of egg-yolk antibody for detection of respiratory syncytial virus in nasal secretions by ELISA.

M Zrein, G Obert, M H van Regenmortel.   

Abstract

Egg-yolk immunoglobulins extracted from the eggs of hens immunized with respiratory syncytial virus (RSV) have been used as a reagent in double sandwich ELISA for detecting RSV in nasal secretions. The sensitivity of virus detection was the same in indirect ELISA, using rabbit anti chicken globulin conjugate, as when biotinylated yolk globulin and labeled avidin were used for detection. The specificity of ELISA for detecting RSV using yolk antibody was similar to that achieved by indirect immunofluorescence using commercial reagents of mammalian origin. Purified immunoglobulin was easily extracted from egg yolk; the amount of globulin present in a single preparation obtained from a batch of ten eggs was sufficient to carry out 10(6) ELISA tests for RSV detection.

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Year:  1986        PMID： 3524512     DOI： 10.1007/bf01317370

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  23 in total

1.  The use of beta-lactamase in enzyme immunoassays for detection of microbial antigens.

Authors:  R H Yolken; S B Wee; M Van Regenmortel
Journal:  J Immunol Methods       Date:  1984-10-12       Impact factor: 2.303

2.  Detection of rotavirus by serological trapping on antibody-coated electron microscope grids.

Authors:  A Nicolaieff; G Obert; M H van Regenmortel
Journal:  J Clin Microbiol       Date:  1980-07       Impact factor: 5.948

3.  Comparison of virus culturing and immunofluorescence for rapid detection of respiratory syncytial virus in nasopharyngeal secretions: sensitivity and specificity.

Authors:  B A Lauer
Journal:  J Clin Microbiol       Date:  1982-08       Impact factor: 5.948

4.  Detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease.

Authors:  H K Sarkkinen; P E Halonen; P P Arstila; A A Salmi
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948

5.  Detection of respiratory syncytial virus in nasopharyngeal secretions by ELISA: comparison with fluorescent antibody technique.

Authors:  A Hornsleth; B Friis; P Andersen; E Brenøe
Journal:  J Med Virol       Date:  1982       Impact factor: 2.327

6.  Detection of a wide spectrum of tobacco mosaic virus strains by indirect enzyme-linked immunosorbent assays (ELISA).

Authors:  M H Van Regenmortel; J Burckard
Journal:  Virology       Date:  1980-10-30       Impact factor: 3.616

7.  Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labeled antibody and peroxidase biotin--avidin complex.

Authors:  R H Yolken; F J Leister; L S Whitcomb; M Santosham
Journal:  J Immunol Methods       Date:  1983-02-11       Impact factor: 2.303

8.  Quantitative microcomplement fixation tests using chicken anti-viral antibody extracted from egg yolk.

Authors:  M H Van Regenmortel; J Burckard
Journal:  J Virol Methods       Date:  1985-07       Impact factor: 2.014

9.  Detection of respiratory syncytial virus in nasopharyngeal secretions by inhibition of enzyme-linked immunosorbent assay.

Authors:  A Hornsleth; E Brenøe; B Friis; F U Knudsen; P Uldall
Journal:  J Clin Microbiol       Date:  1981-11       Impact factor: 5.948

10.  Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques.

Authors:  L Minnich; C G Ray
Journal:  J Clin Microbiol       Date:  1980-09       Impact factor: 5.948
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  3 in total

1.  An enzyme-linked immunosorbent assay using monoclonal antibodies for the detection of respiratory syncytial virus in clinical specimens.

Authors:  G Obert; C Beyer
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

2.  Comparison of monoclonal antibody time-resolved fluoroimmunoassay with monoclonal antibody capture-biotinylated detector enzyme immunoassay for respiratory syncytial virus and parainfluenza virus antigen detection.

Authors:  J C Hierholzer; P G Bingham; R A Coombs; K H Johansson; L J Anderson; P E Halonen
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

3.  Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans.

Authors:  S Hamada; T Horikoshi; T Minami; S Kawabata; J Hiraoka; T Fujiwara; T Ooshima
Journal:  Infect Immun       Date:  1991-11       Impact factor: 3.441
  3 in total

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