Literature DB >> 35218534

Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Cells.

Cassandra R Edgar1, Jimmy D Dikeakos2.   

Abstract

Examining protein-protein interactions provides critical insight into numerous human diseases and infections. Here we describe a protocol for bimolecular fluorescence complementation, which can be used to directly visualize and characterize intracellular protein-protein interactions and ascertain their localization using fluorescence microscopy.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Bimolecular fluorescence complementation; Microscopy; Protein–protein interactions; Subcellular localization

Mesh：

Substances：

Year:  2022        PMID： 35218534     DOI： 10.1007/978-1-0716-2051-9_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation.

Authors:  Chang-Deng Hu; Yurii Chinenov; Tom K Kerppola
Journal:  Mol Cell       Date:  2002-04       Impact factor: 17.970

Review 2.  Protein-protein interactions in human disease.

Authors:  Daniel P Ryan; Jacqueline M Matthews
Journal:  Curr Opin Struct Biol       Date:  2005-08       Impact factor: 6.809

Review 3.  Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells.

Authors:  Tom K Kerppola
Journal:  Annu Rev Biophys       Date:  2008       Impact factor: 12.981

4.  Interactome-transcriptome analysis reveals the high centrality of genes differentially expressed in lung cancer tissues.

Authors:  Shinichiro Wachi; Ken Yoneda; Reen Wu
Journal:  Bioinformatics       Date:  2005-09-27       Impact factor: 6.937

5.  HIV-1 Nef sequesters MHC-I intracellularly by targeting early stages of endocytosis and recycling.

Authors:  Brennan S Dirk; Emily N Pawlak; Aaron L Johnson; Logan R Van Nynatten; Rajesh A Jacob; Bryan Heit; Jimmy D Dikeakos
Journal:  Sci Rep       Date:  2016-11-14       Impact factor: 4.379

6.  Global topological features of cancer proteins in the human interactome.

Authors:  Pall F Jonsson; Paul A Bates
Journal:  Bioinformatics       Date:  2006-07-14       Impact factor: 6.937

7.  Network-based classification of breast cancer metastasis.

Authors:  Han-Yu Chuang; Eunjung Lee; Yu-Tsueng Liu; Doheon Lee; Trey Ideker
Journal:  Mol Syst Biol       Date:  2007-10-16       Impact factor: 11.429

8.  Viral bimolecular fluorescence complementation: a novel tool to study intracellular vesicular trafficking pathways.

Authors:  Brennan S Dirk; Rajesh Abraham Jacob; Aaron L Johnson; Emily N Pawlak; P Craig Cavanagh; Logan Van Nynatten; S M Mansour Haeryfar; Jimmy D Dikeakos
Journal:  PLoS One       Date:  2015-04-27       Impact factor: 3.240

Review 9.  Protein-protein interactions of human viruses.

Authors:  Norman Goodacre; Prajwal Devkota; Eunhae Bae; Stefan Wuchty; Peter Uetz
Journal:  Semin Cell Dev Biol       Date:  2018-07-26       Impact factor: 7.727

10.  HIV-1 Vpu Downregulates Tim-3 from the Surface of Infected CD4+ T Cells.

Authors:  Jérémie Prévost; Cassandra R Edgar; Jonathan Richard; Steven M Trothen; Rajesh Abraham Jacob; Mitchell J Mumby; Suzanne Pickering; Mathieu Dubé; Daniel E Kaufmann; Frank Kirchhoff; Stuart J D Neil; Andrés Finzi; Jimmy D Dikeakos
Journal:  J Virol       Date:  2020-03-17       Impact factor: 5.103
  10 in total

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