| Literature DB >> 35218529 |
Caroline Sodja1, Deborah Callaghan1, Arsalan S Haqqani1, Danica B Stanimirovic1, Willard J Costain1, Anna Jezierski2.
Abstract
Automated high-throughput immunoassays are emerging as reliable analytic techniques for the quantitative detection of proteins from a variety of sample types. Herein, we describe a method using the Protein Simple Wes capillary-based automated immunoassays platform for the quantification of His- and HA-tagged antibody transcytosis across an in vitro transwell blood-brain barrier (BBB) model. Compared to conventional ELISA, fluorescence, and Mass Spec-based detection approaches, Wes provides comparable datasets with additional information regarding size, aggregation, and potential degradation of samples before and after BBB transcytosis. In this chapter, we have benchmarked our Wes technique against ELISA and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), using known BBB crossing (FC5) and non-crossing (A20.1) single domain antibodies.Entities:
Keywords: Antibody transcytosis; Blood-brain barrier; Immunoassay
Mesh：
Substances：
Year: 2022 PMID： 35218529 DOI： 10.1007/7651_2021_456
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745