Ultrastructural study of Candida albicans yeast after application of a ribonuclease.

Electron microscopy was performed on Candida albicans yeast after application of ribonuclease to clear the cytoplasmic background. In conjunction with Thiery's method of highlighting polysaccharide components, this clearing technique, which has not been used since 1959, enabled visualization of the nucleus, the mitochondria, the vacuolar system and another structure which seemed to be the Golgi apparatus. The invaginations of the plasmalemma membrane (or lomasomes) are highly developed and may be partially responsible for transporting material required for development of the cell wall, especially during budding.