Incidence and predictive factors for recurrent clear cell ovarian carcinoma: results from a single center in Thailand.

OBJECTIVE: We aimed to study the incidence and predictive factors of recurrent clear cell ovarian carcinoma (CCC) and evaluate the oncological outcomes after recurrence.
METHODS: This was a retrospective study of 134 CCC cases diagnosed between 2005 and 2020. Clinicopathological data and oncological outcomes were extracted and evaluated. Patients with co-malignancy, mixed pathological type, or incomplete data were excluded. Descriptive statistics, univariate and multivariable analyses, and Kaplan-Meier survival probability estimates were completed. A proportional hazards model was used to assess the association between the prognostic factors with progression-free survival (PFS), overall survival (OS), and post-recurrence survival.
RESULTS: A total of 134 patients with CCC were enrolled. The incidence of recurrent CCC was 33.6% (45/134). The median PFS was 12.8 months (95% confidence interval [CI], 9.66-18.9) in the recurrence group and 3.3 months (95% CI, 1.15-4.4) in the refractory group. Residual tumor from surgical outcome, ascites cytology, and lymphovascular space invasion (LVSI) were independent prognostic factors for PFS. The significant variables were residual tumor (sub-optimal surgery vs. optimal surgery) (hazard ratio [HR], 2.68; 95% CI, 1.48-4.87; P=0.002), ascites cytology (positive vs. negative) (HR, 2.8; 95% CI, 1.58-4.98; P=0.002), and LVSI (positive vs. negative) (HR, 2.14; 95% CI, 1.18-3.86; P=0.04). The median postrecurrence survival was 13.96 months (95% CI, 10.61-26.2) in the recurrence group.
CONCLUSION: CCC has a high rate of recurrence. Sub-optimal surgery, positive ascites cytology, and LVSI indicated a worse prognosis for PFS. Optimal cytoreductive surgery is an important part of primary treatment to improve survival in patients with CCC.

Introduction

Ovarian cancer is the third most common gynecological cancer worldwide [1]. The most common type is serous carcinoma, which accounts for 75–80% of all epithelial ovarian cancers [2]. Clear cell ovarian carcinoma (CCC) is a rare type of ovarian cancer. Among ethnic groups, CCC has a different prevalence. The prevalence of ovarian CCC was 5% in the entire female population, whereas its prevalence in the Asian population has increased to 13–25% [3,4].

Patients with CCC usually present with early stage disease and are younger than those with serous epithelial ovarian carcinoma [5]. Even though patients present with early stage disease, patients with CCC still have a high rate of recurrence [6]. Currently, the treatment of CCC is based on evidence-based studies of epithelial ovarian carcinoma, which is mainly composed of serous ovarian carcinoma and a small percentage of CCC patients. The standard treatment for ovarian carcinoma is surgical staging and platinum-based chemotherapy, which depends on the stage of the disease [7,8]. Current guidelines for the management of epithelial ovarian carcinoma do not distinguish between different histological or molecular subtypes. The response rate of CCC to chemotherapy was lower in the primary and recurrent treatment settings. A previous study reported that <10% of CCC patients were platinum-sensitive, and 1% of patients had platinum-resistant disease [9]. Therefore, it is important to identify the prognostic factors of CCC to establish guidelines for treatment and prognosis evaluation.

Due to the high prevalence of CCC in the Asian population, this study aimed to evaluate the incidence of recurrent CCC, the predictive factors of recurrent disease, and the oncological outcomes after recurrence of the disease.

Materials and methods

A retrospective study was conducted in 134 patients diagnosed with CCC and treated with primary cytoreductive surgery or neoadjuvant chemotherapy followed by interval debulking surgery in the Department of Obstetrics and Gynecology of Songklanagarind Hospital between January 2005 and March 2020. Patients who had co-malignancies, mixed pathological type, received previous chemotherapy or radiotherapy, had incomplete medical records, or were lost to follow-up were excluded. The study was approved by the Research Ethics Committee of the Faculty of Medicine, Prince of Songkla University (REC 63-167-12-4).

The following variables of all patients were reviewed and collected: age at diagnosis, preoperative level of serum cancer antigen 125 (CA 125), stage at diagnosis based on the 2014 International Federation of Gynecology and Obstetrics (FIGO) staging system; primary treatment, tumor size, residual tumor after primary surgery, ascites cytology; lymphovascular space invasion (LVSI), adjuvant treatment, chemotherapy regimen, site of recurrent disease, current disease status, and date of death or last follow-up. The term “sub-optimal surgery” was defined by the presence of a residual tumor from surgery that was greater than 1 cm; an “optimal surgery” was defined by the lack of residual tumors or the presence of a residual tumor from surgery that was smaller than 1 cm. Recurrent disease was defined by recurrence after complete remission from the primary treatment; it was identified via physical examinations, tumor markers, radiological imaging, and/or histological diagnoses. The term “refractory” in this study was used to define a partial response, stable disease, or progression of the disease after primary treatment. Progression-free survival (PFS) was defined as the period from the time of diagnosis to the time of recurrence or progression, the last follow-up, or death from any cause. Overall survival (OS) was defined as the period from the time of diagnosis to the time of last follow-up or death from any cause. Post-recurrence survival was defined as the period from the time of diagnosis of first recurrence or progression to the last follow-up or death from any cause.

The data were processed using R version 3.4.3 (R Foundation for Statistical Computing). Kaplan-Meier analysis was used to determine PFS, OS, and post-recurrence survival. Multivariable analysis was performed using the Cox proportional hazards model to evaluate the independent factors affecting survival. Hazard ratios (HRs) and 95% confidence intervals (CIs) were also calculated. Statistical significance was set at P<0.05.

Results

A total of 134 CCC cases were included and analyzed in this study. The population was divided into three groups: 64 cases (47.8%) with remission of disease, 45 cases (33.6%) with recurrent disease, and 25 cases (18.7%) with refractory disease. In this study, the overall incidence of recurrent CCC was 33.6% (45/134). Patient characteristics at the time of initial diagnosis are shown in Table 1. The mean age of the entire population was 51.5 (standard deviation [SD], 8.6) years. The mean (interquartile range, IQR) initial level of CA 125 was 116 IU/mL (39.2–384.8). Of all patients, 32/134 (23.9%) of CCC cases in this study had normal pretreatment CA 125 levels (<35 IU/mL). The vast majority of 96 patients (71.6%) had early stage disease (stage I to II), and 38 patients (28.4%) had advanced stage disease (stage III to IV). Most patients (96.3%) were treated with primary cytoreductive surgery followed by platinum-based chemotherapy, and 85% of the patients achieved an “optimal surgery”. All patients presented with an average tumor size ranging from 10 to 16 cm. Ascites cytology was positive in 27.6% of patients. LVSI was present in 17.9% of the patients. Of the entire study group, 11/134 (8.2%) had nodal involvement. Most patients (99.3%) received adjuvant platinum-based chemotherapy. None of the patients received radiotherapy or bevacizumab as part of their primary treatment. Significant statistical differences were revealed between the three groups in terms of initial preoperative CA 125 level at the time of diagnosis, FIGO stage, ascites cytology, residual tumor from primary surgical outcome, and lymph node involvement. The median (IQR) CA 125 levels in the remission, recurrence, and refractory groups were 72.5 IU/mL (29–212.8), 157 IU/mL (41–542), and 384 IU/mL (102–871) (P=0.002). Advance-stage disease in the remission, recurrence, and refractory groups was found in 12.5%, 37.8%, and 52% of the patients, respectively (P<0.001). The patients with positive ascites cytology in the remission, recurrence, and refractory groups were 6/64 (9.3%), 20/45 (24.4%), and 11/25 (44%), respectively (P<0.001). The number (percentage) of patients with sub-optimal surgery in the remission, recurrence, and refractory groups were 2/65 (3.1%), 7/45 (15.6%), and 11/25 (44%), respectively (P<0.001). The number (percentage) of patients with positive lymph node involvement in the remission, recurrence, and refractory groups were 0 (0.0%), 8 (17.8%), and 3 (12.0%), respectively (P<0.001).

Table 1

Baseline patient characteristics

Clinicopathological feature	Remission	Recurrence	Refractory	Total	P-value
Total	64 (47.8)	45 (33.6)	25 (18.6)	134 (100.0)
Age at diagnosis	52.5±9.1	50.8±7.9	50±8.5	51.5±8.6	0.372
Age (yr)					0.495
<50	23 (35.9)	20 (44.4)	12 (48.0)	55 (41.0)
≥50	41 (64.1)	25 (55.6)	13 (52.0)	79 (59.0)
Initial CA 125	72.5 (29.0–212.8)	157 (41–542)	384 (102–871)	116 (39.2–384.8)	0.002
CA 125 level mean (IU/mL)					0.066
≤35	20 (31.3)	10 (22.2)	2 (8.0)	32 (23.9)
>35	44 (68.7)	35 (77.8)	23 (92.0)	102 (76.1)
FIGO stage					<0.001
I	28 (43.8)	11 (24.4)	7 (28.0)	46 (34.4)
II	28 (43.8)	17 (37.8)	5 (20.0)	50 (37.3)
III	6 (9.3)	17 (37.8)	12 (48.0)	35 (26.1)
IV	2 (3.1)	0 (0.0)	1 (4.0)	3 (2.2)
FIGO stage					<0.001
Early	56 (87.5)	28 (62.2)	12 (48.0)	96 (71.6)
Advanced	8 (12.5)	17 (37.8)	13 (52.0)	38 (28.4)
Primary treatment					0.710
Primary surgery	61 (95.3)	43 (95.6)	25 (100.0)	129 (96.3)
NACT	3 (4.7)	2 (4.4)	0 (0.0)	5 (3.7)
Surgical outcome					<0.001
Optimal surgery	62 (96.9)	38 (84.4)	14 (56.0)	114 (85.1)
Sub-optimal surgery	2 (3.1)	7 (15.6)	11 (44.0)	20 (14.9)
Tumor size (cm)	12 (10, 16)	13 (10, 17)	12 (10, 14)	12 (10, 16)	0.570
Ascites cytology					<0.001
Negative	41 (64.1)	18 (40.0)	7 (28.0)	66 (49.3)
Positive	6 (9.3)	20 (44.4)	11 (44.0)	37 (27.6)
No data	17 (26.6)	7 (15.6)	7 (28.0)	31 (23.1)
LVSI					0.057
Negative	33 (51.6)	19 (42.2)	10 (40.0)	62 (46.3)
Positive	5 (7.8)	11 (24.4)	8 (32.0)	24 (17.9)
No data	26 (40.7)	15 (33.3)	7 (28.0)	48 (35.8)
Lymph node involvement					<0.001
Positive	0 (0.0)	8 (17.8)	3 (12.0)	11 (8.2)
Negative	43 (67.2)	37 (82.2)	12 (48.0)	92 (68.7)
No data	21 (32.8)	0 (0.0)	10 (32.3)	31 (23.1)
Adjuvant chemotherapy					1.000
No	1 (1.6)	0 (0.0)	0 (0.0)	1 (0.7)
Yes	63 (98.4)	45 (100)	25 (100)	133 (99.3)
Chemotherapy					0.526
No data	0 (0.0)	0 (0.0)	0 (0.0)	1 (0.0)
Platinum-based	63 (100.0)	45 (100.0)	25 (100.0)	133 (100.0)

Values are presented as mean±standard deviation, median (interquartile range), or number (%).

CA 125, cancer antigen 125; FIGO stage, International Federation of Gynecology and Obstetrics stage 2014; NACT, neoadjuvant chemotherapy; LVSI, lymphovascular space invasion.

The median follow-up time of the entire cohort was 40.5 months (95% CI, 15.9–76.1). At the time of analysis, 56/134 (41.8%) died of the disease, and the remaining 78/134 (58.2%) survived. In the group that survived, 60/78 (76.92%) had no evidence of disease and 18/78 (23.07%) continued to live with the disease. Survival curves were estimated using the Kaplan-Meier method. The median PFS was 12.8 months (95% CI, 9.66–18.9) for the recurrence group and 3.3 months (95% CI, 1.15–4.4) for the refractory group (Fig. 1). The median OS values were as follows: not reached, 41.7 months (95% CI, 31.7–51.1), and 15.7 months (95% CI, 13.7–22.3) for the remission, recurrence, and refractory groups, respectively (Fig. 2).

Fig. 1

Kaplan-Meier analysis of progression-free survival according to group.

Fig. 2

Kaplan-Meier analysis of overall survival according to group.

For stages I, II, and III, the median PFS values were 17.58 months (95% CI, 12.8 to not applicable [NA]), 17.74 months (95% CI, 9.6–25.0), and 9.89 months (95% CI, 7.82–16.1), respectively. The 1-year PFS rates for stages I, II, and III were 72.7%, 58.8%, and 41.1%, respectively. The 3-year PFS rates for stages I, II, and III were 9.0%, 5.8%, and 5.8%, respectively. The OS rates at 1, 3, and 5 years were 94.0%, 69.2%, and 57.1%, respectively, for the entire population. For the remission, recurrence, and refractory groups, the OS rates at 1 year were 100%, 97.8%, and 71.8%; at 3 years, they were 96.7%, 60.9%, and 4.91%, respectively, and at 5 years they were 96.7%, 21.3%, and 4.91%, respectively.

In the univariate analysis (Table 2), FIGO stage, surgical outcome, ascites cytology, LVSI, preoperative level of CA 125, and lymph node involvement were found to have significantly poor PFS. Therefore, FIGO stage, surgical outcome, ascites cytology, LVSI, preoperative level of CA 125, and lymph node involvement were entered into the multivariate PFS analysis. The Cox multivariate analysis showed that residual tumor from surgical outcome, LVSI, and ascites cytology were independent prognostic factors for PFS. The significant variables were residual tumor (>1 cm vs. <1 cm or no residual tumor) (HR, 2.68; 95% CI, 1.48–4.87; P=0.002), LVSI (positive vs. negative) (HR, 2.14; 95% CI, 1.18–3.86; P=0.04), and ascites cytology (positive vs. negative) (HR, 2.8; 95% CI, 1.58–4.98; P=0.002).

Table 2

Univariate and multivariate analyses for factors associated with PFS

Factor	Univariate		Multivariate
	HR (95% CI)	P-value	HR (95% CI)	P-value
Age	0.98 (0.95–1.01)	0.16
Stage
Early (I+II)	1
Advance (III+IV)	3.21 (1.58–5.19)	<0.001
Primary treatment
NACT	1
Surgery	0.66 (0.16–2.69)	0.535
Surgical outcome
Optimal surgery	1		1
Sub-optimal surgery	4.54 (1.01–3.51)	<0.001	2.68 (1.48–4.87)	0.002
Ascites cytology
Negative	1		1
Positive	3.79 (2.21–6.49)	<0.001	2.80 (1.58–4.98)	0.002
LVSI
Negative	1		1
Positive	2.57 (1.44–4.61)	0.004	2.14 (1.18–3.86)	0.04
CA 125 (IU/mL)
≤35	1		1
>35	1.88 (1.01–3.50)	0.034	1.56 (0.83–2.94)	0.149
Lymph node involvement
Negative	1
Positive	3.30 (1.68–6.47)	0.001

PFS, progression-free survival; HR, hazard ratio; CI, confidence interval; NACT, neoadjuvant chemotherapy; LVSI, lymphovascular space invasion; CA 125, cancer antigen 125.

The post-recurrence survival period in the recurrence group was 13.96 months (95% CI, 10.61–26.2), and this was 7.8 months (95% CI, 4.14–11.1) in the refractory group (Fig. 3). Most patients in the recurrence group (29/45, 64.4%) had multiple-site recurrence and 13/45 (28.9%) had single-site recurrence (all 13 patients had recurrent tumors in the pelvis). Another three out of 45 (6.7%) patients had nodal recurrence. The first patient had a single nodal recurrence in the right pelvic region. The second had four nodes above the left renal vein, and the last one had multiple nodal recurrences: seven nodes at both groins and pelvic and para-aortic lymph nodes (Supplementary Table 1). Multiple-site recurrence had a poorer prognosis than single-site recurrence and nodal recurrence (Supplementary Table 2).

Fig. 3

Kaplan-Meier analysis of post-recurrence survival according to group.

Five patients had a prolonged post-recurrence survival of more than 36 months (Table 3). One patient had no evidence of disease, three patients were living with the disease, and one patient died from the disease 56.9 months after recurrence. The age range of the patients was 48–59 years. They were initially diagnosed with FIGO stage II and underwent primary cytoreductive surgery followed by chemotherapy. The time of recurrence was 11–67 months. The pattern of recurrence in these patients was either single-site or nodal site recurrence. The treatment in these cases after recurrence was mainly chemotherapy. One patient was treated with tumor debulking surgery followed by chemotherapy, and the post-recurrence survival in this case was 105.1 months. Since these patients were classified as having platinum-sensitive recurrent ovarian cancer, all received reinduction chemotherapy with paclitaxel and carboplatin.

Table 3

Descriptive analysis of five patients with prolonged post-recurrence survival >36 months

No.	Age (yr)	Stage	Primary treatment	Time to recurrence (months)	Site of recurrence	Treatment for recurrence	Post-recurrence survival (months)	Disease status
1	59	II	Cytoreductive surgery+CMT	30.8	Single-site pelvis	Debulking surgery+CMT	105.1	NED
2	54	II	Cytoreductive surgery+CMT	67.5	Single-site pelvis	CMT	56.9	Died from disease
3	48	II	Cytoreductive surgery+CMT	42.1	Single-site pelvis	CMT	39.4	AWD
4	49	II	Cytoreductive surgery+CMT	32.6	Pelvic lymph node	CMT	49.0	AWD
5	55	II	Cytoreductive surgery+CMT	11.5	Single-site pelvis	CMT	42.9	AWD

CMT, chemotherapy; NED, no evidence of disease; AWD, alive with disease.

Discussion

The incidence recurrence rate of CCC was 33.6%, which was consistent with previous studies that reported rates of 26.9–30.3% [9-11]. The incidence of recurrence was 29.1% in early stage disease and 44.7% in advanced stage disease. These results were similar to those of a study by Hogen et al. [9] that reported recurrence rates of 26% in early stage disease. However, the recurrence rate in advanced stage disease was 67% [9], which is much higher than that reported in this study. Even though the percentage of patients that achieved an optimal surgery was relatively equivalent, this may have occurred because this study did not include patients with stage IV disease. Hreshchyshyn et al. [12] reported a recurrence rate of only 6% in stage I epithelial ovarian carcinoma after surgery and treatment with chemotherapy. In contrast, Sugiyama et al. [13] found that stage IC CCC had a higher rate (37%) and shorter time of recurrence compared with serous adenocarcinoma.

The patients had a mean age at the time of diagnosis of 51 years. This was consistent with the results of a previous study in which the median age in patients with CCC was 49–51 years old, which was significantly younger than that of patients with serous carcinoma of the ovary (55 vs. 64 years) [14-16]. The preoperative level of CA 125 was less than 35 IU/mL (normal) in 23% of all patients in this study. A previous study also reported a normal CA 125 level in 19.4–39.1% of patients with CCC [17,18]. Currently, the European Society for Medical Oncology and European Society of Gynaecologial Oncology consensus conference recommendations on ovarian cancer indicate that CA 125 is not a reliable marker in epithelial ovarian carcinoma types other than high-grade serous carcinoma [7]. Cases of suspected or diagnosed CCC with a negative level of CA 125 should be further investigated using alternative serum markers to improve early diagnosis and prognosis, as well as monitoring after treatment. Most CCC patients (71.4%) presented with early stage disease, which is consistent with a previous study that reported early stage disease in 66.4–79% of patients [3,9,19]. In contrast, most patients (83.2%) with serous carcinoma presented with advanced stage disease [3].

The median PFS in this study was 12.8 months (95% CI, 9.66–18.9) in the recurrence group. Most patients (77.7%, 35/45) experienced recurrence within the first 2 years after the primary treatment. A previous study reported a median PFS of 14.5–15.5 months [11,20]. This study found that early stage disease had a median PFS that was greater than that for advanced stage disease. In this study, the median PFS durations for stages I, II, and III were 17.5, 17.7, and 9.8 months. This study included patients with optimal and sub-optimal surgical outcomes, while the study by Kajiyama et al. [11] included only CCC patients with complete resection outcomes; therefore, the PFS durations the aforementioned study might be different and longer than in this study.

Data on predictive factors for recurrent disease in CCC remain scarce. Most data on predictive factors for recurrence of epithelial ovarian carcinoma resulted from serous ovarian carcinoma. Lee et al. [6] reported that early stage (stage I), endometriosis, and optimal debulking were favorable prognostic factors for PFS. A previous retrospective study provided evidence that residual tumor status is an independent prognostic factor for patients with CCC [21]. Additionally, a retrospective study showed that LVSI was associated with a decreased PFS in stage I CCC, and they concluded that patients with stage I CCC showing LVSI may benefit from receiving postoperative chemotherapy [22]. Similarly, in this study, residual tumor from surgical outcome, LVSI, and positive ascites cytology indicated a worse prognosis for PFS. Unfortunately, CCC had a lower response rate to platinum-based chemotherapy (11.1%) than serous adenocarcinoma (72.5%) [13]. Maximal cytoreductive surgery should be attempted to delay recurrence and prolong survival in patients with CCC. Currently, the recommendation for adjuvant chemotherapy in CCC is still based on data from epithelial ovarian cancer trials, which are composed of a small number of CCC cases. They suggested that adjuvant chemotherapy was based mainly on the FIGO stage. However, this study showed that LVSI and positive ascites cytology indicated a worse prognosis for PFS. Therefore, further investigations on the values of other clinicopathological factors should be considered in personalized adjuvant therapy.

We found that multiple-site recurrence was the most common pattern of recurrence in CCC (64.4%), and diffuse peritoneal involvement was the most common. This is consistent with a previous study that reported that 62% of patients had multiple-site recurrence [9]. Post-recurrence survival in this study was 13.96 months. Previous studies reported median post-recurrence survivals of 14.0–16.5 months [11,19,23]. Platinum sensitivity and primary stage were reported as independent prognostic factors for recurrent or persistent CCC, whereas chemotherapy regimens (paclitaxel plus carboplatin versus irinotecan plus cisplatin) did not demonstrate a significant difference [23]. Our investigations revealed that single-site and nodal site recurrences indicated a favorable prognosis for post-recurrence survival. It has been reported that surgical resection combined with systemic chemotherapy might improve the oncological outcome in patients with single-site recurrent CCC [9,20]. Kondo et al. [23] reported survival rates of tumor reduction surgery during recurrence. The median post-progression survival in the tumor reduction surgery group was significantly longer than in the non-tumor reduction surgery group (26.5 NA vs. 12.9 months) (HR, 0.23; 95% CI, 0.09–0.63; P=0.002) [23]. In agreement with this study, a patient who underwent debulking surgery followed by systemic chemotherapy had significantly longer post-recurrence survival than patients who received only systemic chemotherapy. The role of debulking surgery in single-site or nodal site recurrent CCC should be further evaluated to achieve promising oncological outcomes.

The limitations of this study are its retrospective study design and incomplete data. None of the patients in this study received bevacizumab as adjuvant therapy. Hence, the value of anti-angiogenic agents could not be evaluated. Nonetheless, this research was a single-institute study that discussed homogeneous management and had a sufficient follow-up period. The analysis provided useful information for counselling patients and their relatives.

Prospective studies should be attempted to provide more complete and accurate data. A prospective clinical trial on new molecular targeted therapies could result in better treatment options for patients with CCC.

In summary, the recurrence rate was 33.6% in patients with CCC. Sub-optimal surgery, LVSI, and positive ascites cytology indicated a worse prognosis for PFS. Optimal cytoreductive surgery is an important part of primary treatment to improve survival in patients with CCC. The treatment of recurrent disease should be individualized for patients, and optimal treatment should be offered to improve oncological outcomes.