Literature DB >> 3519137

Molecular cloning and expression in Escherichia coli of the cDNA coding for rabbit tumor necrosis factor.

H Ito, S Yamamoto, S Kuroda, H Sakamoto, J Kajihara, T Kiyota, H Hayashi, M Kato, M Seko.   

Abstract

cDNA clones containing the entire sequence of the precursor of rabbit tumor necrosis factor (TNF) were identified from a cDNA library prepared using poly(A)+RNA of lipopolysaccharide-induced rabbit alveolar cells. Synthetic oligodeoxynucleotides based on the amino acid sequence of rabbit TNF were selected by RNA blot hybridization and used as probes to screen this library. The DNA sequence and the amino acid sequence deduced from it showed very high homology to the reported DNA and amino acid sequences of human TNF. A plasmid containing the tac promoter and cDNA sequence coding for the 154-amino-acid sequence of mature rabbit TNF protein was constructed and expressed in Escherichia coli. The polypeptide produced had the characteristics of purified rabbit serum TNF and caused a necrotic response in a transplanted syngeneic tumor in mice.

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Year:  1986        PMID： 3519137     DOI： 10.1089/dna.1986.5.149

Source DB:  PubMed          Journal:  DNA        ISSN： 0198-0238


  11 in total

1.  Primary structure of ovine tumor necrosis factor alpha cDNA.

Authors:  A J Young; J B Hay; J Y Chan
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

2.  Adaptation to bacterial lipopolysaccharide controls lipopolysaccharide-induced tumor necrosis factor production in rabbit macrophages.

Authors:  J C Mathison; G D Virca; E Wolfson; P S Tobias; K Glaser; R J Ulevitch
Journal:  J Clin Invest       Date:  1990-04       Impact factor: 14.808

3.  Signalling pathway of tumor necrosis factor in normal and tumor cells.

Authors:  N Watanabe; H Neda; Y Ohtusuka; H Sone; N Yamauchi; M Maeda; H Kuriyama; Y Niitsu
Journal:  Cancer Immunol Immunother       Date:  1989       Impact factor: 6.968

Review 4.  Cachectin/tumor necrosis factor: an endogenous mediator of shock and inflammation.

Authors:  B Beutler; A Cerami
Journal:  Immunol Res       Date:  1986       Impact factor: 2.829

5.  Fungal beta-glucan interacts with vitronectin and stimulates tumor necrosis factor alpha release from macrophages.

Authors:  E J Olson; J E Standing; N Griego-Harper; O A Hoffman; A H Limper
Journal:  Infect Immun       Date:  1996-09       Impact factor: 3.441

6.  Ocular inflammatory effects of intravitreally-injected tumor necrosis factor.

Authors:  J T Rosenbaum; E L Howes; R M Rubin; J R Samples
Journal:  Am J Pathol       Date:  1988-10       Impact factor: 4.307

7.  Expression of recombinant feline tumor necrosis factor is toxic to Escherichia coli.

Authors:  C M Otto; F Niagro; X Su; C A Rawlings
Journal:  Clin Diagn Lab Immunol       Date:  1995-11

8.  The genes for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) are tandemly arranged on chromosome 17 of the mouse.

Authors:  S A Nedospasov; B Hirt; A N Shakhov; V N Dobrynin; E Kawashima; R S Accolla; C V Jongeneel
Journal:  Nucleic Acids Res       Date:  1986-10-10       Impact factor: 16.971

9.  Role of TNF alpha in the induction of antigen induced arthritis in the rabbit and the anti-arthritic effect of species specific TNF alpha neutralising monoclonal antibodies.

Authors:  J Lewthwaite; S Blake; T Hardingham; R Foulkes; S Stephens; L Chaplin; S Emtage; C Catterall; S Short; A Nesbitt
Journal:  Ann Rheum Dis       Date:  1995-05       Impact factor: 19.103

10.  Participation of tumor necrosis factor in the mediation of gram negative bacterial lipopolysaccharide-induced injury in rabbits.

Authors:  J C Mathison; E Wolfson; R J Ulevitch
Journal:  J Clin Invest       Date:  1988-06       Impact factor: 14.808
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