A novel control strategy to strengthen nitrogen removal from domestic wastewater through eliminating nitrite oxidizing bacteria in a plug-flow process.

In this study, intermittent aeration strategy was investigated in a plug-flow reactor on real municipal wastewater. Over 200 days of operation, the total inorganic nitrogen (TIN) removal efficiency of 72.43 ± 7.56% was achieved with a total aerobic hydraulic retention time in the range 2.4-3.0 h under a low C/N ratio of 3.19. The batch tests showed that the activity of nitrite oxidizing bacteria (NOB) was effectively inhibited, and simultaneous nitrification and denitrification via nitrite were observed under double intermittent aeration mode. The Illumina MiSep sequencing revealed that the relative abundance of the Nitrospira as the only detected NOB, decreased from 2.22% (day 0) to 0.91% (day 207) at the genus level. Overall, this study provides a new strategy for NOB suppression to strengthen nitrogen removal from low C/N domestic wastewater through the continuous process.